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Series GSE75902 Query DataSets for GSE75902
Status Public on Dec 31, 2015
Title Biogenesis and function of tRNA fragments during sperm maturation and fertilization in mammals (IVF)
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Parental dietary conditions can influence the metabolic traits of offspring. In mice, paternal consumption of low protein diet alters cholesterol and lipid metabolism of progeny. Here, we examine RNA species expressed in male reproductive tissues of mice. Protein restriction leads to altered levels of multiple small RNAs in mature sperm, as well as throughout the male reproductive tract, with decreased levels of let-7 family members and increased levels of 5’ fragments of tRNA-Gly isoacceptors. Intriguingly, tRNA fragments are scarce in the testis, but their levels increase in sperm during post-testicular maturation in the epididymis. We find that epididymosomes – extracellular vesicles which fuse with sperm during epididymal transit – exhibit RNA payloads closely matching those of mature sperm, and can deliver tRNA fragments to immature sperm in vitro both in mouse and in bull. Finally, we show that tRNA-Gly-GCC fragments play a role in repressing genes associated with the endogenous retroelement MERVL, both in ES cells and in preimplantation embryos. Our results shed light on small RNA biogenesis during post-testicular sperm maturation, and link tRNA fragments to regulation of endogenous retroelements active in the early embryo.
 
Overall design IVF was carried out using oocytes from females fed Control diet (C) and sperm from males fed Control diet or Low Protein diet (LP). Zygotes were then developed 2 cell (2C), 4 cell (4C), 8 cell (8C), Morula (M) or Blastocyst (B) embryonic developmental stages when single embryo RNA seq was carried out to study gene expression changes.
 
Contributor(s) Shea J, Derr A, Rando O
Citation(s) 26721685
Submission date Dec 10, 2015
Last update date May 15, 2019
Contact name Upasna Sharma
E-mail FNU.Upasna@umassmed.edu
Phone 508 856 6337
Organization name Umass Med School
Department BMP
Lab LRB
Street address 364 Plantation Street
City Worcester
State/province MA
ZIP/Postal code 01605
Country USA
 
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (280)
GSM1969757 p01_01
GSM1969758 p01_02
GSM1969759 p01_03
This SubSeries is part of SuperSeries:
GSE75622 Biogenesis and function of tRNA fragments during sperm maturation and fertilization in mammals
Relations
BioProject PRJNA305650
SRA SRP067241

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Supplementary file Size Download File type/resource
GSE75902_Sharma_Rando_TableS7.xlsx 19.3 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data is available on Series record

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