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Series GSE75791 Query DataSets for GSE75791
Status Public on Dec 11, 2015
Title Assessment of functional overlap between mouse Dux and human DUX4 in vitro and in vivo
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary We report both DUX4 and Dux toxicity depend upon their ability to bind DNA and activate transcription. Chromatin immunoprecipitation of V5 epitope tagged human DUX4 and mouse Dux was performed in human myoblasts was analyzed using ChIP-Seq to identify their subsequent binding sites. We found that DUX4 and Dux bind 4-8% of identical sequences, while majority of the binding sites are unique to either DUX4 or Dux. Although small, this overlap could be due to their conserved abilioty to regualte primordial pathways that were essential for life and therefore maintained in both proteins despite their separate evolutionary paths.
Overall design We performed ChIP-Seq analysis of human myoblasts transfected with plasmids encoding either epitope tagged human DUX4 (1 sample) and mouse Dux (1 sample). Illumina sequencing libraries were prepared from the ChIP and Input DNA, then resulting DNA libraries were quantified and sequenced and aligned to the human genome (hg19).
Contributor(s) Harper SQ, Eidahl JO, Giesige CR
Citation(s) 28173143
Submission date Dec 08, 2015
Last update date May 15, 2019
Contact name Scott Q Harper
Phone (614) 355-2893
Organization name Nationwide Children's Hospital
Department Gene Therapy
Lab Harper
Street address 700 Children's Drive
City Columbus
State/province Ohio
ZIP/Postal code 43205
Country USA
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (4)
GSM1967859 mDux-V5_V5
GSM1967860 mDux-V5_Input
GSM1967861 DUX4-V5_V5
BioProject PRJNA305358
SRA SRP067117

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Supplementary file Size Download File type/resource
GSE75791_RAW.tar 684.5 Mb (http)(custom) TAR (of BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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