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| Status |
Public on Dec 07, 2016 |
| Title |
Sutherlanida extract inhibits Hedgehog signaling target genes |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Sutherlandia frutescens (L) R. Br. (Sutherlandia) is a South African botanical that is traditionally used to treat a variety of health conditions, infections and diseases, including cancer.
We hypothesized Sutherlandia might act through Gli/ Hedgehog (Hh)-signaling in prostate cancer cells and used RNA-Seq transcription profiling to profile gene expression in TRAMPC2 murine prostate cancer cells with or without Sutherlandia extracts.
We found 50% of Hh-responsive genes can be repressed by Sutherlandia ethanol extract, including the canonical Hh-responsive genes Gli1 and Ptch1 as well as newly distinguished Hh-responsive genes Hsd11b1 and Penk.
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| Overall design |
Using mouse prostate cancer TRAMPC2 cells, we treated TRAMPC2 cells with Hh ligand conditioned medium (Hh-CM), 8ug/ml or 80ug/ml Sutherlandia, co-treatment of Hh-CM with 8ug/ml or 80ug/ml Sutherlandia for 8 or 24 hrs and performed gene expression profiling using RNA-Seq. Control experiment were set up for both 8hr and 24hr treatment by treating the cells with ethanol only. We have done 2 biological replicate for each treatment.
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| Contributor(s) |
Yuan L, Dennis LB |
| Citation(s) |
26710108 |
| |
| Submission date |
Dec 07, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Yuan Lu |
| Organization name |
Xiphophorus Genetic Stock Center
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| Street address |
university drive
|
| City |
San Marcos |
| State/province |
TX |
| ZIP/Postal code |
78666 |
| Country |
USA |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA305284 |
| SRA |
SRP067034 |
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