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Series GSE75544 Query DataSets for GSE75544
Status Public on Dec 31, 2015
Title Sperm tsRNAs contribute to intergenerational inheritance of an acquired metabolic disorder
Organism Mus musculus
Experiment type Non-coding RNA profiling by high throughput sequencing
Expression profiling by high throughput sequencing
Methylation profiling by high throughput sequencing
Summary Increasing evidences indicate diet-induced metabolic disorder could be paternally inherited, but the exact sperm epigenetic carrier remains unclear. Here, in a paternal high-fat diet (HFD) mouse model, we revealed that a highly enriched subset of sperm small RNAs (30-34 nt) that derived from the 5’ halves of tRNAs (tsRNAs), exhibit changes in both expression profiles and RNA modifications. Injection of sperm tsRNAs from HFD male but not synthetic tsRNAs lacking RNA modifications, into normal zygotes generated metabolic disorders in the F1 offspring. Injection of HFD sperm tsRNAs derails gene expression in both early embryos and islets of F1 offspring, enriched in metabolic pathways, but unrelated to DNA methylation at CpG-enriched region. Collectively, we uncover sperm tsRNAs as a type of “epigenetic carrier” that mediate intergenerational inheritance of acquired traits.
Overall design Mature sperm small-RNA profiles between High-fat-diet (HFD) and Normal-diet (ND) males; Transcriptional profiles of 8-cell embryos and balstocysts that developed from zygotes that injected with sperm RNAs from HFD vs ND males. Transcriptional profiles and RRBS profiles of islets of F1 offsrping that generated from zygotes that injected with sperm RNAs from HFD vs ND males.
Contributor(s) Shi J, Chen Q, Duan E, Zhou Q
Citation(s) 26721680
Submission date Dec 01, 2015
Last update date May 15, 2019
Contact name Junchao Shi
Organization name University of California, Riverside
Department Division of Biomedical Sciences, School of Medicine
Street address 900 University Ave
City Riverside
State/province CA
ZIP/Postal code 92521
Country USA
Platforms (2)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (16)
GSM1958911 sperm normal diet [ncRNA-seq]
GSM1958912 sperm high fat diet [ncRNA-seq]
GSM1958913 sperm fragment 30-40nt normal diet [ncRNA-seq]
BioProject PRJNA304514
SRA SRP066841

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Supplementary file Size Download File type/resource
GSE75544_RAW.tar 68.5 Mb (http)(custom) TAR (of TXT, WIG)
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Raw data are available in SRA
Processed data provided as supplementary file

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