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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jan 03, 2016 |
| Title |
Inhibition of p300 Lysine Acetyltransferase activity by Luteolin reduces tumor growth in head and neck squamous cell carcinoma (HNSCC) xenograft mouse model [mRNA] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Chromatin acetylation is attributed with distinct functional relevance with respect to gene expression in normal and diseased conditions thereby leading to a topical interest in the concept of epigenetic modulators and therapy. We report here the identification and characterization of the acetylation inhibitory potential of an important dietary flavonoid, luteolin. Luteolin was found to inhibit p300 acetyltransferase with competitive binding to the acetyl CoA binding site. Luteolin treatment in a xenografted tumor model of head and neck squamous cell carcinoma (HNSCC), led to a dramatic reduction in tumor growth within 4 weeks corresponding to a decrease in histone acetylation. Cells treated with luteolin exhibit cell cycle arrest and decreased cell migration. Luteolin treatment led to an alteration in gene expression and miRNA profile including up-regulation of p53 induced miR-195/215, let7C; potentially translating into a tumor suppressor function. It also led to down-regulation of oncomiRNAs such as miR-135a, thereby reflecting global changes in the microRNA network. Furthermore, a direct correlation between the inhibition of histone acetylation and gene expression was established using chromatin immunoprecipitation on promoters of differentially expressed genes. A network of dysregulated genes and miRNAs was mapped along with the gene ontology categories, and the effects of luteolin were observed to be potentially at multiple levels: at the level of gene expression, miRNA expression and miRNA processing.
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| Overall design |
Cells treated with luteolin exhibit cell cycle arrest and decreased cell migration. Luteolin treatment led to an alteration in gene expression and mRNA profile
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| Contributor(s) |
Selvi RB, Swaminathan A, Chatterjee S, Shanmugam MK, Li F, Ramakrishnan GB, Siveen KS, Chinnathambi A, Emam ZM, Alharbi SA, Basha J, Bhat A, Vasudevan M, Dharmarajan A, Sethi G, Kundu TK |
| Citation(s) |
26517526 |
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| Submission date |
Nov 16, 2015 |
| Last update date |
Apr 23, 2018 |
| Contact name |
madavan vasudevan |
| E-mail(s) |
madavan.vasudevan@gmail.com
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| Phone |
+91-9845093355
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| Organization name |
Theomics International Private Limited
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| Street address |
Kasturi Nagar
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| City |
Bangalore |
| State/province |
Karnataka |
| ZIP/Postal code |
560043 |
| Country |
India |
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| Platforms (1) |
| GPL16699 |
Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Feature Number version) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA305010 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE75029_RAW.tar |
73.4 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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