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Series GSE74938 Query DataSets for GSE74938
Status Public on Sep 13, 2016
Title RNA-seq profiling of mouse PGC (Mus musculus)
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary PGCs undergo two distinct stages of demethylation before reaching a hypomethylated ground state at E13.5. Stage 1 occurs between E7.25- E9.5 in which PGCs experience a global loss of cytosine methylation. However, discreet loci escape this global loss of methylation and between E10.5-E13.5, stage 2 of demethylation takes place. In this stage these loci are targeted by Tet1 and Tet2 leading to the loss of the remaining methylation and resulting in the epigenetic ground state. Our data shows that Dnmt1 is responsible for maintaining the methylation of loci that escape stage 1 demethylation, and that it functions in a UHRF1 independent manner. Our data further demonstrates that when these loci lose methylation prior to stage 2 it results in early activation of the meiotic program, which leads to precocious differentiation of the germ line resulting in a decreased pool of PGCs in the embryo and subsequent infertility in adult mice.
Overall design Examination of transcription of Mouse PGCs
Contributor(s) Hargan-Calvopina J, Taylor S, Cook H, Hu Z, Lee SA, Yen M, Chiang Y, Chen P, Clark AT
Citation(s) 27618282
Submission date Nov 12, 2015
Last update date May 15, 2019
Contact name Pao-Yang Chen
Organization name Academia Sinica
Department Institute of Plant and Microbial Biology
Lab Pao-Yang Chen
Street address 128 Sec. 2, Academia Rd, Nankang,
City Taipei
ZIP/Postal code 115
Country Taiwan
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (37)
GSM1938056 E13.5 Female DCKO rep1
GSM1938057 E13.5 Female DCKO rep2
GSM1938058 E13.5 Female DCKO rep3
BioProject PRJNA301971
SRA SRP066097

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Supplementary file Size Download File type/resource
GSE74938_RPKM_table_E10_5.txt.gz 468.3 Kb (ftp)(http) TXT
GSE74938_RPKM_table_E11_5.txt.gz 1.1 Mb (ftp)(http) TXT
GSE74938_female_gene_exp.diff.gz 814.3 Kb (ftp)(http) DIFF
GSE74938_male_gene_exp.diff.gz 807.8 Kb (ftp)(http) DIFF
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Raw data are available in SRA
Processed data are available on Series record

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