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| Status |
Public on Dec 23, 2015 |
| Title |
Expression data from Pseudomonas aeruginosa ∆sbrR and ∆sbrIR mutants versus wild type |
| Platform organism |
Pseudomonas aeruginosa |
| Sample organism |
Pseudomonas aeruginosa PAO1 |
| Experiment type |
Expression profiling by array
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| Summary |
SbrI and SbrR are an extracytoplasmic function sigma factor and its cognate anti-sigma factor, respectively. To identify the SbrIR regulon, we measured gene expression in wild type PAO1 , PAO1 ∆sbrR, and PAO1 ∆sbrIR mutants using microarrays.
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| Overall design |
WT PAO1 pPSV38 (empty vector), PAO1 ∆sbrR pPSV38, PAO1 ∆sbrR pSbrR, and PAO1 ∆sbrIR pPSV38 were grown to mid-log. RNA was extracted and reverse transcribed into cDNA. The cDNA was biotinylated and hybridized to Pseudomonas aeruginosa Affymetrix microarrays.
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| Contributor(s) |
McGuffie BA, Dove SL |
| Citation(s) |
26620262 |
| Submission date |
Nov 11, 2015 |
| Last update date |
Jul 06, 2016 |
| Contact name |
Bryan McGuffie |
| Organization name |
Boston Children's Hospital
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| Department |
Infectious Diseases
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| Lab |
Dove
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| Street address |
300 Longwood Ave
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
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| Platforms (1) |
| GPL84 |
[Pae_G1a] Affymetrix Pseudomonas aeruginosa Array |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA301901 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE74917_RAW.tar |
5.2 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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