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Series GSE74767 Query DataSets for GSE74767
Status Public on Aug 24, 2016
Title A developmental coordinate of the spectrum of pluripotency among mice, monkeys, and humans
Organisms Macaca fascicularis; Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The epiblast (EPI) is the origin of all somatic and germ cells in mammals, and of the spectrum of pluripotent stem cells (PSCs) in vitro. To explore the ontogeny of human/primate pluripotency, we performed comprehensive single-cell RNA sequencing for pre- and post-implantation EPI development in cynomolgus monkeys. Here we show that after specification in the blastocysts [embryonic day (E)7], cyEPI undergoes major transcriptome changes upon implantation. Thereafter, cyEPI, while generating gastrulating cells (~E13), maintains its transcriptome relatively stably over a week, retaining a unique set of pluripotency genes while acquiring properties for “neuron differentiation.” h/cyPSCs show the highest similarity to post-implantation late cyEPI (~E17), which, despite co-existing with gastrulating cells, bears characteristics of pre-gastrulating mouse EPI (E5.5) and epiblast-like cells (EpiLCs) in vitro. These findings not only reveal divergence/coherence of EPI development, but also identify a developmental coordinate of the spectrum of pluripotency among key species, providing a basis for better regulation of human pluripotency in vitro.
Overall design Single cell transcriptome analysis of cynomolgus monkey embryo E6-17 and mouse embryo E4.5 - E6.5 as well as of cynomogus monkey embryonic stem cells (ESCs) and mouse ESC, epiblast like cells (EpiLCs), using SC3-seq technology.
Contributor(s) Nakamura T, Okamoto I, Sasaki K, Yabuta Y, Iwatani C, Tsuchiya H, Yamamoto T, Saitou M
Citation(s) 27556940, 28649393
Submission date Nov 06, 2015
Last update date May 15, 2019
Contact name Yukihiro Yabuta
Organization name Kyoto University, Graduate school of medicine
Department Anatomy and Cell Biology
Street address Yoshida-Konoe-cho, Sakyo-ku
City Kyoto
State/province Kyoto
ZIP/Postal code 606-8501
Country Japan
Platforms (2)
GPL15907 AB 5500xl Genetic Analyzer (Mus musculus)
GPL19944 AB 5500xl Genetic Analyzer (Macaca fascicularis)
Samples (492)
GSM1932150 C6FF_MS11T85
GSM1932151 C6FF_MS11T86
GSM1932152 C6FF_MS11T87
BioProject PRJNA301445
SRA SRP065899

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE74767_SC3seq_Cy_ProcessedData.txt.gz 17.5 Mb (ftp)(http) TXT
GSE74767_SC3seq_Ms_ProcessedData.txt.gz 4.5 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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