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| Status |
Public on May 03, 2016 |
| Title |
Effect of Cited2 knockdown on global transcript expression in Rcho-1 cell differentiation |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We had previously discovered that the transcription factor Cited2 was highly induced during trophoblast differentiation. In this study, we used an lentiviral shRNA strategy to decrease Cited2 expression in Rcho-1 trophoblast cells. A RNA-seq approach was used to determine global transcript differences inRcho-1 knockdown cells compared to control cells.
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| Overall design |
Rcho-1 cells transduced with control shRNAs were used as controls. Cells transduced with shRNAs targetingCited2 were used as treatment.Cells were differentiated for 8 days and the analyses were done.
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| Contributor(s) |
Dhakal P, Imakawa K, Soares MJ |
| Citation(s) |
26917451 |
| |
| Submission date |
Nov 06, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Pramod Dhakal |
| E-mail(s) |
pdhakal@kumc.edu
|
| Phone |
913-588-5690
|
| Organization name |
University of Kansas Medical Center
|
| Department |
Pathology and Laboratory Medicine
|
| Street address |
3901 Rainbow Boulevard
|
| City |
Kansas City |
| State/province |
Kansas |
| ZIP/Postal code |
66160 |
| Country |
USA |
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| Platforms (1) |
| GPL14844 |
Illumina HiSeq 2000 (Rattus norvegicus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA301403 |
| SRA |
SRP065879 |
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