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Series GSE74628 Query DataSets for GSE74628
Status Public on Nov 04, 2015
Title Mouse primary myeloid dendritic cell transcriptome response to a wild type infectious clone of West Nile virus (WNVMT) and mutant virus (WNVE218A).
Organism Mus musculus
Experiment type Expression profiling by array
Summary The purpose is to obtain samples for mRNA analysis in primary mouse myeloid dendritic cells infected with wild type West Nile virus (WNVMT) and mutant virus (WNVE218A).
 
Overall design Mouse (C57Bl/6J) primary amyeloid dendritic cells (D10 after seeding of leukocytes from bone marrow) were seeded at 2 x 10^5 cells per well of non-tc treated 12-well plate in 0.8 mL R10 DC media after infection at a multiplicity of infection of 500. Infected samples were collected in triplet; time-matched mocks were collected in triplet in parallel with infected samples. Time points: 1, 8, 12 and 24 h post-infection.

There were two independent biological replicates of the entire procedure. This study (WDC010) is a biological replicate of another (WDC011; GSE75222).
 
Contributor(s) Thackray L, Diamond M, Stratton K, Waters KM, Eisfeld AJ, Kawaoka Y
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Nov 03, 2015
Last update date Nov 01, 2017
Contact name Natalie Heller
E-mail natalie.heller@pnnl.gov
Organization name PNNL
Street address 902 Battelle Blvd.
City Richland
ZIP/Postal code 99354
Country USA
 
Platforms (1)
GPL11202 Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version)
Samples (33)
GSM1924616 WDC010_mockulum_MOI500_1h_1_RNA
GSM1924617 WDC010_mockulum_MOI500_1h_2_RNA
GSM1924618 WDC010_mockulum_MOI500_1h_3_RNA
This SubSeries is part of SuperSeries:
GSE65575 Modeling Host Responses to Understand Severe Human Virus Infections
Relations
BioProject PRJNA301036

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE74628_RAW.tar 71.4 Mb (http)(custom) TAR (of TXT)
Raw data provided as supplementary file
Processed data included within Sample table

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