The purpose is to obtain samples for mRNA analysis in primary mouse myeloid dendritic cells infected with wild type West Nile virus (WNVMT) and mutant virus (WNVE218A).
Mouse (C57Bl/6J) primary amyeloid dendritic cells (D10 after seeding of leukocytes from bone marrow) were seeded at 2 x 10^5 cells per well of non-tc treated 12-well plate in 0.8 mL R10 DC media after infection at a multiplicity of infection of 500. Infected samples were collected in triplet; time-matched mocks were collected in triplet in parallel with infected samples. Time points: 1, 8, 12 and 24 h post-infection.
There were two independent biological replicates of the entire procedure. This study (WDC010) is a biological replicate of another (WDC011; GSE75222).