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Series GSE74599 Query DataSets for GSE74599
Status Public on Nov 04, 2015
Title ChIP-seq after immunoprecipitation with either IgG or SOX2 antibodies in murine genetically modified stem cells overexpressing the RAX gene (CCE-Rx cells)
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary In order to identify target genes of the SOX2 transcription factor, we tried to identify SOX2 binding site in murine genetically modified stem cells overexpressing the RAX gene (CCE-Rx cells) by ChIP-seq. SOX2 gene mutations are involved in anophthalmia and our hypothesis was that target genes of SOX2 transcription factor may also be candidate to be involved in ocular development.
Overall design High throughput sequencing after chromatin immunoprecipitation using either an antibody against our target transcription factor (SOX2) or using control antobodies (IgG). These experiments were performed on only one immunoprecipitation with each antibody.
Contributor(s) Chassaing N
Citation(s) 26893459
Submission date Nov 03, 2015
Last update date May 15, 2019
Contact name Nicolas Chassaing
Organization name CHU Toulouse
Department Service de Génétique Médicale
Street address Place du Dr Baylac
City Toulouse
ZIP/Postal code 31059
Country France
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (2)
GSM1923656 IgG ChIP-Seq
GSM1923657 SOX2 ChIP-Seq
This SubSeries is part of SuperSeries:
GSE74600 Transcriptomic analysis and ChIP-seq in CCE-Rx cells to identify SOX2 transcription factor target genes
BioProject PRJNA300946
SRA SRP065664

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE74599_ChIP-seq_results_SOX2_versus_IgG.xls.gz 515.4 Kb (ftp)(http) XLS
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Raw data are available in SRA
Processed data are available on Series record

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