|
| Status |
Public on Nov 03, 2015 |
| Title |
Small nucleolar RNA and microRNA expression data from an MS patient's and a healthy control's PBMC cultures stimulated with phytohemagglutinin. |
| Platform organism |
synthetic construct |
| Sample organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
Multiple sclerosis is a common inflammatory and degenerative disease that causes neurological disability. It affects young adults and its prevalence is higher in women. The most common form is manifested as a series of acute episodes of neurological disability (relapses) followed by a recovery phase (remission). Recently, non-coding RNAs have emerged as new players in transcriptome regulation, and in turn, they could have a significant role in MS pathogenesis. In this context, our aim was to investigate the involvement of microRNAs and snoRNAs in the relapse-remission dynamics of MS in peripheral blood leucocytes, to shed light on the molecular and regulatory mechanisms that underlie this complex process. With this approach, we found that a subset of small non-coding RNAs (sncRNA) is altered in relapse and remission, revealing unexpected opposite changes that are sex dependent. Furthermore, we found that a relapse-related miRNA signature regulated general metabolism processes in leucocytes, and miRNA altered in remission are involved in the regulation of innate immunity. We observed that sncRNA dysregulation is different in relapse and remission leading to differences in transcriptome regulation, and that this process is sex dependent. In conclusion, relapse and remission have a different molecular background in men and women.
|
| |
|
| Overall design |
PBMC samples from a MS patient and a healthy control cultured with and without stimulation with phytohemagglutinin.
|
| |
|
| Contributor(s) |
Muñoz-Culla M, Otaegui D |
| Citation(s) |
26831009 |
| |
| Submission date |
Nov 02, 2015 |
| Last update date |
May 02, 2017 |
| Contact name |
Maider Muñoz-Culla |
| E-mail(s) |
maider.munoz@ehu.eus
|
| Organization name |
Biodonostia Institute
|
| Street address |
Paseo Dr.Begiristain s/n
|
| City |
San Sebastian |
| ZIP/Postal code |
20014 |
| Country |
Spain |
| |
|
| Platforms (1) |
| GPL19117 |
[miRNA-4] Affymetrix Multispecies miRNA-4 Array |
|
| Samples (4)
|
| GSM1923349 |
cultured PBMC from MS patient, no stimulated |
| GSM1923350 |
cultured PBMC from MS patient stimulated with PHA |
| GSM1923351 |
cultured PBMC from healthy control, no stimulated |
| GSM1923352 |
cultured PBMC from healthy control stimulated with PHA |
|
| This SubSeries is part of SuperSeries: |
| GSE74586 |
SncRNA (microRNA & snoRNA) expression in leucocytes from multiple sclerosis relapse and remission and cultured peripheral blood mononuclear cells |
|
| Relations |
| BioProject |
PRJNA300874 |
Less...
More...