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Series GSE74463 Query DataSets for GSE74463
Status Public on Oct 30, 2015
Title Altered lipid metabolism in the aging kidney identified by three layered omic analysis
Organism Mus musculus
Experiment type Expression profiling by array
Summary Aging-related diseases and their comorbidities affect the life quality of a constantly growing proportion of our population. Age-associated changes of kidney structure and function are considerable contributors to the dramatically increased incidence of chronic kidney disease world-wide which has been identified to be a prominent cardiovascular risk factor. In order to detect molecular mechanisms involved in kidney aging we analyzed gene expression profiles of kidneys from adult and aged wild-type mice by a three-layered omics strategy. To this end, transcriptomic, proteomic and targeted lipidomic profiles of young and aged mice were generated and integrated. Transcriptome and proteome analyses revealed differential expression of genes involved primarily in lipid metabolism and immune response. Additional lipidomic analyses uncovered significant age-related differences in the total amount of phosphatidylethanolamines, phosphatidylcholines and sphingomyelins as well as in subspecies of phosphatidylserines and ceramides, while total ceramide levels remained unchanged. By integration of these datasets we identified Aldh1a1, a key enzyme in vitamin A metabolism specifically expressed in the medullary ascending limb, as one of the most prominent upregulated proteins in old kidneys. Moreover, ceramidase Asah1 was highly expressed in aged kidneys, consistent with a decrease in ceramide C16.
In summary, our data suggest that changes in lipid metabolism are involved in the process of kidney aging and in the development of chronic kidney disease.
Overall design 14 weeks females (4 replicates); 96 weeks females (5 replicates)
Contributor(s) Braun F, Rinschen MM, Bartels V, Frommolt P, Habermann B, Hoeijmakers JH, Schumacher B, Dollé ME, Müller R, Benzing T, Schermer B, Kurschat CE
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Submission date Oct 29, 2015
Last update date Mar 04, 2019
Contact name Peter Frommolt
Organization name CECAD
Street address Joseph-Stelzmannstr. 26
City Cologne
ZIP/Postal code 50931
Country Germany
Platforms (1)
GPL6246 [MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version]
Samples (9)
GSM1921098 14_weeks_female_wildtype_replicate_1
GSM1921099 14_weeks_female_wildtype_replicate_2
GSM1921100 14_weeks_female_wildtype_replicate_3
BioProject PRJNA300498

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Supplementary file Size Download File type/resource
GSE74463_RAW.tar 37.9 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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