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| Status |
Public on Jan 07, 2016 |
| Title |
GATA-1 and heme regulate the erythroid cell transcriptome. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Alas2 gene encodes the rate-limiting enzyme in heme biosynthesis. CRISPR/Cas9-mediated ablation of two Alas2 intronic cis-elements strongly reduced GATA-1-induced Alas2 transcription, heme biosynthesis, and GATA-1 regulation of other vital constituents of the erythroid cell transcriptome. Bypassing Alas2 function in Alas2 cis-element-mutant (double mutant) cells by providing its catalytic product 5-aminolevulinic acid (5-ALA) rescued heme biosynthesis and the GATA-1-dependent genetic network. We discovered a GATA factor- and heme-dependent circuit that establishes the erythroid cell transcriptome.
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| Overall design |
G1E-ER-GATA-1 WT and double mutant cells were examined. Untreated WT, beta-estradiol-treated WT, beta-estradiol-treated double-mutant, and beta-estradiol/5-ALA-treated double-mutant cells were subjected to RNA-seq.
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| Contributor(s) |
Tanimura N, Dewey CN, Bresnick EH |
| Citation(s) |
26698166 |
| Submission date |
Oct 26, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Kyle Hewitt |
| E-mail(s) |
KJHEWITT@WISC.EDU
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| Organization name |
University of Wisconsin
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| Street address |
1111 Highland Ave.
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| City |
Madison |
| State/province |
Wisconsin |
| ZIP/Postal code |
53705 |
| Country |
USA |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (12)
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| GSM1919044 |
Estradiol-treated WT cells, Replicate 1 |
| GSM1919045 |
Estradiol-treated WT cells, Replicate 2 |
| GSM1919046 |
Estradiol-treated WT cells, Replicate 3 |
| GSM1919047 |
Estradiol-treated double mutant cells, Replicate 1 |
| GSM1919048 |
Estradiol-treated double mutant cells, Replicate 2 |
| GSM1919049 |
Estradiol-treated double mutant cells, Replicate 3 |
| GSM1919050 |
Estradiol/5-ALA-treated double mutant cells, Replicate 1 |
| GSM1919051 |
Estradiol/5-ALA-treated double mutant cells, Replicate 2 |
| GSM1919052 |
Estradiol/5-ALA-treated double mutant cells, Replicate 3 |
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| Relations |
| BioProject |
PRJNA299837 |
| SRA |
SRP065286 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE74371_RAW.tar |
7.8 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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