 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Oct 26, 2015 |
| Title |
Transcriptomic Comparison of Neuronal Development Stages using Induced Pluripotent Stem Cells from Bipolar Disorder Patients |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Fibroblasts from patients with Type I bipolar disorder (BPD) and their unaffected siblings were obtained from an Old Order Amish pedigree with a high incidence of BPD and reprogrammed to induced pluripotent stem cells (iPSCs). Established iPSCs were subsequently differentiated into neuroprogenitors (NPs) and then to neurons. Transcriptomic microarray analysis was conducted on RNA samples from iPSCs, NPs and neurons matured in culture for either 2 weeks (termed early neurons, E) or 4 weeks (termed late neurons, L). Global RNA profiling indicated that BPD and control iPSCs differentiated into NPs and neurons at a similar rate, enabling studies of differentially expressed genes in neurons from controls and BPD cases. Significant disease-associated differences in gene expression were observed only in L neurons. Specifically, 328 genes were differentially expressed between BPD and control L neurons including GAD1, glutamate decarboxylase 1 (2.5 fold) and SCN4B, the voltage gated type IV sodium channel beta subunit (-14.6 fold). Quantitative RT-PCR confirmed the up-regulation of GAD1 in BPD compared to control L neurons. Gene Ontology, GeneGo and Ingenuity Pathway Analysis of differentially regulated genes in L neurons suggest that alterations in RNA biosynthesis and metabolism, protein trafficking as well as receptor signaling pathways GSK3β signaling may play an important role in the pathophysiology of BPD.
|
| |
|
| Overall design |
Samples for each of four iPSCs, NPs and neurons matured in culture for either 2 weeks (termed early neurons, E) or 4 weeks (termed late neurons, L) were analyzed
|
| |
|
| Contributor(s) |
Kim KH, Liu J, Sells Galvin RJ, Dage JL, Egeland JA, Smith RC, Merchant KM, Paul SM |
| Citation(s) |
26554713 |
| Submission date |
Oct 26, 2015 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Jiangang Liu |
| E-mail(s) |
liu_jiangang@lilly.com
|
| Phone |
317-433-0033
|
| Organization name |
Eli Lilly and Company
|
| Department |
Tailored Therapeutics
|
| Street address |
893 S Delaware St
|
| City |
Indianapolis |
| State/province |
IN |
| ZIP/Postal code |
46225 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
|
| Samples (28)
|
|
| Relations |
| BioProject |
PRJNA299812 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE74358_RAW.tar |
146.7 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...