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Series GSE74243 Query DataSets for GSE74243
Status Public on Jul 01, 2016
Title Genome wide gene expression during lung development in three inbred mouse strains
Organism Mus musculus
Experiment type Expression profiling by array
Summary To better understand the temporal dynamics of gene expression during normal murine lung development we characterized global gene expression at 26 time points in three common inbred strains of mice (A/J, C57BL/6J, and C3H/HeJ). The data set provides a unique resource for identifying patterns of gene expression changes during normal lung development and for investigating the developmental origins of respiratory disease.
The transcriptional profiles generated for lung development in three inbred strains of the laboratory mouse revealed concordance with pre-natal stages of lung development defined by anatomy and morphology. The genomic data support the view that the postnatal alveolar development is composed of 4 distinct molecular stages. The data revealed strain specific differences in the expression of genes related to respiratory cell differentiation, pulmonary innervation, metabolic pathway interactions, and immune system function.
Overall design Mouse lungs or whole embryos (e09.5 only) were surgically dissected at 26 time points across all six canonical stages of lung development (embryonic-EMB, pseudoglandular-PSG, canalicular-CAN, saccular-SAC, alveolar-ALV, homeostatic-HOM) to identify transcriptional profiles associated with 9 molecular divisions of lung development (embryonic-EMB, pseudoglandular-PSG, canalicular-CAN, saccular-SAC, alveolar1-ALV1, alveolar2-ALV2, alveolar3-ALV3, alveolar4-ALV4, homeostatic-HOM). Lung tissue from three or four embryos collected at e11.5 or e12.5, respectively, was pooled for each sample to obtain sufficient RNA for array analysis. Tissue from at least three animals was collected for each time point (biological replicates). Only male mice were used in the study.
Contributor(s) Bult C, Beauchemin K, Wells J
Citation(s) 27602285
Submission date Oct 21, 2015
Last update date Sep 30, 2016
Contact name Carol Bult
Phone 207-288-6324
Organization name The Jackson Laboratory
Street address 600 Main Street
City Bar Harbor
State/province ME
ZIP/Postal code 04609
Country USA
Platforms (1)
GPL20894 [MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [CDF: MoGene10stv1_Mm_ENSG v.19.0.0]
Samples (216)
GSM1915262 Embryo_AJ_e09.5_r1
GSM1915263 Embryo_AJ_e09.5_r2
GSM1915264 Embryo_AJ_e09.5_r3
BioProject PRJNA299588

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Supplementary file Size Download File type/resource
GSE74243_Probes_BultLung2015.tsv.gz 20.6 Mb (ftp)(http) TSV
GSE74243_RAW.tar 857.4 Mb (http)(custom) TAR (of CEL)
GSE74243_matrix_RMAlog2expressionvalues.txt.gz 22.9 Mb (ftp)(http) TXT
Processed data are available on Series record

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