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Series GSE74155 Query DataSets for GSE74155
Status Public on Jul 27, 2016
Title Single-cell analysis of allelic gene expression in pluripotency, differentiation and X-chromosome inactivation
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We sequenced the mRNAs of embryonic stem cells (ESCs) cultured in different conditions. The two lines M (male) and F (female) used in this study were derived from E4 blastocysts of the same cross between a C57BL/6J (Mus musculus domesticus) and CAST/EiJ (Mus castaneus) male. mESCs were cultured in 2i and LIF as the ground state condition or in serum and LIF as the conventional condition. Epistem cell lines were also generated from the two lines by culturing them with Activin A and FGF2. In order to study more advanced development, we differentiated the two mESC lines through embryonic body formation to postmitotic motor neurons using retinoic acid and the smoothened agonist SAG. This differentiation process also results in the derivation of several types of interneurons. We picked single cells from all different conditions and generated sequencing libraries using the Smart-seq2 and Tn5 protocol. For simplicity, we designate the different condition as ES2i, ES, Epi and Neuron from hereon. We also obtained preimplantation inner cell mass and epiblast cells from E3.5 ICM (inner cell mass) and E4.5 blastocysts of the crossbred mice (male CAST/EiJ × female C57BL/6J) as well as postimplantation epiblast cells from E5.5 embryos of C57BL/6J mice
Overall design Examination of gene expression profile in individual male and female embryonic stem cell lines along developmental progression
Contributor(s) Deng Q, Chen G
Citation(s) 27486082
Submission date Oct 19, 2015
Last update date May 15, 2019
Contact name Qiaolin Deng
Organization name Karolinska Institutet
Department Cell and Molecular Biology
Lab Developmental Biology
Street address Berzelius väg 35
City Stockholm
ZIP/Postal code 17165
Country Sweden
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (617)
GSM1911848 ES_2i_130
GSM1911849 ES_2i_131
GSM1911850 ES_2i_132
BioProject PRJNA299180
SRA SRP064979

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Supplementary file Size Download File type/resource
GSE74155_Blastocyst_genes_fpkm.txt.gz 2.1 Mb (ftp)(http) TXT
GSE74155_ES2i_ES_Epi_neuron_genes_fpkm.txt.gz 20.0 Mb (ftp)(http) TXT
GSE74155_Epinew_genes_fpkm_table.txt.gz 2.0 Mb (ftp)(http) TXT
GSE74155_Postimplan_genes_fpkm.txt.gz 3.9 Mb (ftp)(http) TXT
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Processed data are available on Series record

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