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| Status |
Public on Jun 27, 2007 |
| Title |
The influence of the deletion of Serum Response Factor in B cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Serum response factor (SRF), a MADS-box transcription factor, is essential for murine embryonic development and for the function of muscle cells and neurons. SRF and its transcriptional co-factors are broadly expressed. To determine the in vivo role of SRF in developing lymphocytes we specifically inactivated the murine Srf gene during T or B cell development using lymphocyte-specific Cre transgenic mouse lines. T cell-specific Srf deletion led to a severe block in thymocyte development at the transition from double to single positive stage. The few residual T cells detectable in the periphery retained at least one functional Srf allele, thereby demonstrating the importance of SRF in T cell development. In contrast, deletion of Srf in developing B cells did not interfere with the growth and survival of B cells in general, yet led to a complete loss of marginal zone B cells and a marked reduction of the CD5+ B cell subset. Our study also revealed a contribution of SRF to the expression of the surface molecules IgM, CD19, and the chemokine receptor 4 in B lymphocytes.
Keywords: Genetic modification
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| Overall design |
Comparison of the gene expression profile between murine wildtype IgM+IgD+ cells and srf-deficient IgM+IgD+ cells isolated from spleens
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| Contributor(s) |
Fleige A, Geffers R, Schippers A |
| Citation(s) |
17591768 |
| Submission date |
Mar 30, 2007 |
| Last update date |
Jan 08, 2019 |
| Contact name |
Robert Geffers |
| E-mail(s) |
robert.geffers@helmholtz-hzi.de
|
| Phone |
+49 531-6181-3058
|
| Organization name |
HCI - Helmholtz Centre for Infection Research
|
| Department |
Dep. Molecular Bacteriology
|
| Lab |
Genome Analytics
|
| Street address |
Inhoffenstr. 7
|
| City |
Braunschweig |
| ZIP/Postal code |
38124 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL339 |
[MOE430A] Affymetrix Mouse Expression 430A Array |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA100381 |
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