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Series GSE74033 Query DataSets for GSE74033
Status Public on Oct 12, 2016
Title Estrogen receptor α promotes breast cancer by reprogramming cell metabolism [ChIP-seq]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Estrogen receptor α (ERα) is a key regulator of breast growth and breast cancer development. However, the role of ERα in metabolic reprogramming, a hallmark of cancer, is not well documented. In this study, using an integrated approach combining genome-wide mapping of chromatin bound ERα with estrogen induced transcript and metabolic profiling, we demonstrate that ERα reprograms metabolism upon estrogen stimulation, including changes in aerobic glycolysis, nucleotide and amino acid synthesis, and choline metabolism. We show, for the first time, that the ERα target gene choline phosphotransferase 1 (CHPT1) plays an essential role in estrogen induced increases in phosphatidylcholine (PtdCho) levels and that CHPT1 promotes tumorigenesis and proliferation. Furthermore, we show that CHPT1 is overexpressed in tumors compared to normal breast. We also demonstrate that ERα promotes aerobic glycolysis through increased expression of glycolytic genes. In conclusion, this study highlights the importance of ERα for metabolic alterations in breast cancer cells. Furthermore, overexpression of the ERα target CHPT1 in breast cancer supports its potential as a therapeutic target.
Overall design Examination of ERα DNA binding sites in T47D, a breast cancer cell line.
Contributor(s) Jia M, Andreassen T, Moestue SA, Sinha I, Gao H, Bathen TF, Zao C, Dahlman-Wright K
Citation(s) 27457520
Submission date Oct 14, 2015
Last update date May 15, 2019
Contact name Karin Dahlman-Wright
Organization name Karolinska Institutet
Department Department of Biosciences and Nutrition
Lab Medicinaren 25/Neo
Street address Blickagången 16
City Huddinge
ZIP/Postal code 14157
Country Sweden
Platforms (1)
GPL9052 Illumina Genome Analyzer (Homo sapiens)
Samples (2)
GSM1908536 DNA input_T47D
GSM1908537 ERα_ChIPseq_E2_T47D
This SubSeries is part of SuperSeries:
GSE74034 Estrogen receptor α promotes breast cancer by reprogramming cell metabolism
BioProject PRJNA298778
SRA SRP064826

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Supplementary file Size Download File type/resource
GSE74033_Min_T47D_E2_vs_input_peaks.bed.gz 183.2 Kb (ftp)(http) BED
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