GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE74001 Query DataSets for GSE74001
Status Public on Dec 23, 2015
Title Mitochondrial Membrane Potential Identifies Cells with Enhanced Stemness for Cellular Therapy
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Long-term survival and antitumor immunity of adoptively transferred CD8+ T cells is dependent on their metabolic fitness, but approaches to isolate therapeutic T cells based on metabolic features are not well established. Here we utilized a lipophilic cationic dye tetramethylrhodamine methyl ester (TMRM) to identify and isolate metabolically robust T cells based on their mitochondrial membrane potential (ΔΨm). Comprehensive metabolomic and gene expression profiling demonstrated global features of improved metabolic fitness in low-ΔΨm-sorted CD8+ T cells. Transfer of these low-ΔΨm T cells was associated with superior long-term in vivo persistence and an enhanced capacity to eradicate established tumors compared with high-ΔΨm cells. Use of ΔΨm-based sorting to enrich for cells with superior metabolic features was observed in CD8+, CD4+ T cell subsets, and long-term hematopoietic stem cells. This metabolism-based approach to cell selection may be broadly applicable to therapies involving the transfer of HSC or lymphocytes for the treatment of viral-associated illnesses and cancer.
Overall design mRNA-Sequencing of gene expression profiles in mouse CD8 cells (TMRM high and TMRM low).
Contributor(s) Sukumar M, Liu J, Mehta GU, Patel SJ, Roychoudhuri R, Crompton JG, Klebanoff CA, JI Y, Li P, Yu Z, Whitehill GD, Clever D, Eil RL, Palmer D, Mitra S, Rao M, Keyvanfer K, Schrump D, Wang E, Marincola F, Gattinoni L, Leonard WJ, Muranski P, Finkel T, Restifo NP
Citation(s) 26674251
Submission date Oct 14, 2015
Last update date May 15, 2019
Contact name Peng Li
Organization name NIH
Department NHLBI
Street address 9000 Rockville Pike
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (2)
GSM1907934 TMRM Low
GSM1907936 TMRM High
BioProject PRJNA298695
SRA SRP064796

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE74001_RAW.tar 1.0 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap