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Series GSE73994 Query DataSets for GSE73994
Status Public on Apr 14, 2017
Title Overexpression of c-Myc antagonises transcriptional output of the androgen receptor in prostate cancer [ChIP-Seq]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary c-Myc overexpression LNCaP cells were treated with R1881 or R1881+Doxycycline (to induce c-Myc overexpression) and ChIP-seq studies were performed using antibodies against c-Myc, AR, H3K4me1, H3K4me3, H3K27ac and H3K27m3
Prostate cancer is the most common non-cutaneous cancer in men. The androgen receptor (AR) a ligand-activated transcription factor, constitutes the main drug target for advanced cases of the disease. However, a variety of other transcription factors and signalling networks have been shown to be altered in patients and to influence AR activity. The oncogenic transcription factor c-Myc has been studied extensively in multiple malignancies, but its impact on AR activity in prostate cancer remains elusive. In this study we assessed the impact of clinically relevant levels of c-Myc overexpression on AR activity and transcriptional output. We found that c-Myc and the AR share a substantial amount of binding sites, which exhibit enhancer-like characteristics. Interestingly, c-Myc overexpression altered global AR chromatin occupancy and antagonised a subset of androgen-induced genes. Furthermore, c-Myc overexpression modified histone marks, most notably H3K4me1 and H3K27me3. Lastly, we validated the antagonistic relationship between c-Myc and two AR target genes, KLK3 and GNMT, in patient samples.
Overall design ChIP-seq study for c-Myc, AR, H3K4me1, H3K4me3, H3K27ac and H3K27m3 in a c-Myc overexpression LNCaP prostate cell line model (LNCaP MYC) treated with R1881 or R1881+Doxycycline
Contributor(s) Barfeld SJ, Urbanucci A, Fazli L, Rennie PS, Yegnasubramanian V, de Marzo AM, Thiede B, Harri IM, Hicks JL, Mills IG
Citation(s) 28412251
Submission date Oct 14, 2015
Last update date May 15, 2019
Contact name Alfonso Urbanucci
Organization name Oslo University Hospital
Department Insitute for Cancer Research, Dept. of Tumor Biology
Lab Urbanucci Lab
Street address Postbox 4950 Nydalen
City Oslo
ZIP/Postal code 0424
Country Norway
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (20)
GSM1907199 AR_R1881_1
GSM1907200 AR_R1881_2
GSM1907201 AR_R1881_Dox_1
This SubSeries is part of SuperSeries:
GSE73995 Overexpression of c-Myc antagonises transcriptional output of the androgen receptor in prostate cancer
BioProject PRJNA298682
SRA SRP064791

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE73994_RAW.tar 10.3 Gb (http)(custom) TAR (of BIGWIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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