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| Status |
Public on Feb 15, 2016 |
| Title |
Genome-wide MAF1-dependent regulation of RNA polymerase III transcription [ChIP-Seq] |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
In higher eukaryotes, an important mechanism to tune translation in different tissues and conditions is mTORC1-dependent regulation of tRNAs transcription by RNA polymerase III (Pol III), as the relative amount of tRNAs tightly coordinates with the translational needs of the cell. mTORC1 contributes to regulate protein synthesis through its direct substrate MAF1, which functions as a negative regulator of Pol III transcription in response to stimuli such as serum starvation or rapamycin treatment. Here, we applied ChIP-seq to examine the Pol III occupancy profile in human fibroblasts and report evidence of a genome wide, MAF1-dependent coordinated response to favorable or stress growth conditions. Strikingly, while a set of genes is extremely responsive in terms of Pol III binding, other genes are mostly unperturbed, yet associated with transcriptionally engaged polymerases as revealed by nascent EU-labeled RNA-seq (neuRNA-seq). As shown by DamIP-seq, the responsiveness of a subset of genes is tightly connected to the rapid and transient interaction of MAF1 with DNA-bound Pol III.
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| Overall design |
We performed duplicate ChIP-seq experiments for the Rpc4 (POLR3D) subunit of RNA polymerase III in IMR90hTert cells grown in the presence of fetal bovine serum (FBS), serum starved (SS), serum starved and treated with insulin (SS+I), serum starved and treated with insulin and rapamycin (SS+R+I). Additional ChIP-seq profiles were generated in cells treated with MAF1 siRNAs and serum starved. MAF1 binding was addressed by DamIP-seq, using two replicates per clone of IMR90hTert cells expressing HA-tagged MAF1-DamK9A (2 different clones) or EGFP-DamK9A (2 different clones). To monitor dynamic transcription profiles we did neusRNA-seq in IMR90hTert cells EU-labeled or mock (DMSO)-labeled. For both DamIP-seq and neusRNA-seq, cells were either unperturbed or serum starved.
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| Contributor(s) |
Orioli A, Praz V, l'Hote P, Hernandez N |
| Citation(s) |
26941251 |
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| Submission date |
Oct 13, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Nicolo Riggi |
| Organization name |
CHUV
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| Department |
Département de Pathologie Expérimentale
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| Lab |
Institut universitaire de pathologie
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| Street address |
Bugnon 25
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| City |
Lausanne |
| State/province |
VD |
| ZIP/Postal code |
1011 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (22)
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| GSM1906345 |
ChIP-seq Rpc4 IMR90hTert cells, (FBS) rep1 |
| GSM1906346 |
ChIP-seq Rpc4 IMR90hTert cells, (FBS) rep2 |
| GSM1906347 |
ChIP-seq Rpc4 IMR90hTert cells, (SS) rep1 |
| GSM1906348 |
ChIP-seq Rpc4 IMR90hTert cells, (SS) rep2 |
| GSM1906349 |
ChIP-seq Rpc4 IMR90hTert cells, (SS_I) rep1 |
| GSM1906350 |
ChIP-seq Rpc4 IMR90hTert cells, (SS_I) rep2 |
| GSM1906351 |
ChIP-seq Rpc4 IMR90hTert cells, (SS_R_I) rep1 |
| GSM1906352 |
ChIP-seq Rpc4 IMR90hTert cells, (SS_R_I) rep2 |
| GSM1906353 |
INPUT DNA IMR90hTert cells, (FBS) rep1 |
| GSM1906354 |
INPUT DNA IMR90hTert cells, (FBS) rep2 |
| GSM1906355 |
INPUT DNA IMR90hTert cells, (SS) rep1 |
| GSM1906356 |
INPUT DNA IMR90hTert cells, (SS) rep2 |
| GSM1906357 |
INPUT DNA IMR90hTert cells, (SS_I) rep1 |
| GSM1906358 |
INPUT DNA IMR90hTert cells, (SS_I) rep2 |
| GSM1906359 |
INPUT DNA IMR90hTert cells, (SS_R_I) rep1 |
| GSM1906360 |
INPUT DNA IMR90hTert cells, (SS_R_I) rep2 |
| GSM1906361 |
ChIP-seq Rpc4 IMR90hTert cells, #NEG_siRNA |
| GSM1906362 |
ChIP-seq Rpc4 IMR90hTert cells, #1MAF1_siRNA |
| GSM1906363 |
ChIP-seq Rpc4 IMR90hTert cells, #2MAF1_siRNA |
| GSM1906364 |
INPUT DNA IMR90hTert cells, #NEG_siRNA |
| GSM1906365 |
INPUT DNA IMR90hTert cells, #1MAF1_siRNA |
| GSM1906366 |
INPUT DNA IMR90hTert cells, #2MAF1_siRNA |
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| This SubSeries is part of SuperSeries: |
| GSE73936 |
Genome-wide MAF1-dependent regulation of RNA polymerase III transcription |
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| Relations |
| BioProject |
PRJNA298536 |
| SRA |
SRP064729 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE73926_RAW.tar |
29.5 Mb |
(http)(custom) |
TAR (of BEDGRAPH) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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