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| Status |
Public on Oct 16, 2015 |
| Title |
Global Gene Expression of Methicillin-resistant Staphylococcus aureus USA300 During Human and Mouse Infection |
| Platform organism |
Staphylococcus aureus subsp. aureus USA300_FPR3757 |
| Sample organism |
Staphylococcus aureus subsp. aureus USA300 |
| Experiment type |
Expression profiling by array
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| Summary |
Little is known about the expression of methicillin-resistant Staphylococcus aureus (MRSA) genes during infection conditions. Here, we described the transcriptome of the clinical MRSA strain USA300 derived from human cutaneous abscesses, and compared it with USA300 bacteria derived from infected kidneys in a mouse model. Remarkable similarity between the transcriptomes allowed us to identify genes encoding multiple proteases and toxins, and iron- and peptide-transporter molecules, which are upregulated in both infections and are likely important for establishment of infection. We also showed that disruption of the global transcriptional regulators agr and sae prevents in vivo upregulation of many toxins and proteases, protecting mice from lethal infection dose, and hinting at the role of these transcriptional regulators in the pathology of MRSA infection.
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| Overall design |
Staphylococcous aureus USA300 were derived from C57Bl/6 mice Mouse kidney abscesses and from human patient cutaneous abscesses. Control wild-type Staphylococcus aureus USA300 samples were taken from 4 hours' culture in TSB. The Mouse, Human, and Control cRNA were Cy5 labeled. A pool of RNA isolated from USA300 bacteria, from various in vitro culture conditions [early log phase (2 h), mid log phase (4 h), post exponential phase (8 h), and stationary phase (20 h) in TSB; mixed at equal ratios] to ensure broad gene expression, was used as reference RNA and was Cy3 labeled.
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| Web link |
http://www.ncbi.nlm.nih.gov/pubmed/24286981
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| Contributor(s) |
Date SV, Modrusan Z, Lawrence M, Morisaki JH, Toy K, Shah IM, Kim J, Park S, Xu M, Basuino L, Chan L, Zeitschel D, Chambers HF, Tan M, Brown EJ, Diep BA, Hazenbos WL |
| Citation(s) |
24286981 |
| Submission date |
Oct 07, 2015 |
| Last update date |
Oct 22, 2017 |
| Contact name |
Mark McCreary |
| E-mail(s) |
mccrearm@gene.com
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| Phone |
6502254332
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| Organization name |
Genentech
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| Department |
Bioinformatics
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| Street address |
1 DNA Way, MS 93
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| City |
South San Francisco |
| State/province |
California |
| ZIP/Postal code |
94080 |
| Country |
USA |
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| Platforms (1) |
| GPL20994 |
Agilent-022825 S.aureus_020409_4x44K |
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| Samples (16)
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| GSM1904025 |
Staphylococcus aureus USA300 derived from Mouse Kidney Abscess Agilent_252282510013_S01_GE2_107_Sep09_1_2 |
| GSM1904026 |
Staphylococcus aureus USA300 derived from Mouse Kidney Abscess Agilent_252282510013_S01_GE2_107_Sep09_1_3 |
| GSM1904027 |
Staphylococcus aureus USA300 derived from Mouse Kidney Abscess Agilent_252282510013_S01_GE2_107_Sep09_1_4 |
| GSM1904028 |
Staphylococcus aureus USA300 derived from Mouse Kidney Abscess Agilent_252282510014_S01_GE2_107_Sep09_1_1 |
| GSM1904029 |
Staphylococcus aureus USA300 derived from Mouse Kidney Abscess Agilent_252282510014_S01_GE2_107_Sep09_1_2 |
| GSM1904030 |
Staphylococcus aureus USA300 derived from Mouse Kidney Abscess Agilent_252282510014_S01_GE2_107_Sep09_1_3 |
| GSM1904031 |
Staphylococcus aureus USA300 derived from Mouse Kidney Abscess Agilent_252282510014_S01_GE2_107_Sep09_1_4 |
| GSM1904032 |
Staphylococcus aureus USA300 derived from Mouse Kidney Abscess Agilent_252282510015_S01_GE2_107_Sep09_1_1 |
| GSM1904033 |
Staphylococcus aureus USA300 derived from Mouse Kidney Abscess Agilent_252282510015_S01_GE2_107_Sep09_1_2 |
| GSM1904034 |
Staphylococcus aureus USA300 derived from Mouse Kidney Abscess Agilent_252282510015_S01_GE2_107_Sep09_1_3 |
| GSM1904035 |
Staphylococcus aureus USA300 derived from Human Skin Pus Agilent_252282510020_S01_GE2_107_Sep09_1_3 |
| GSM1904036 |
Staphylococcus aureus USA300 derived from Human Skin Pus Agilent_252282510021_S01_GE2_107_Sep09_1_3 |
| GSM1904037 |
Staphylococcus aureus USA300 4 hour Log Control Agilent_252282510021_S01_GE2_107_Sep09_1_4 |
| GSM1904038 |
Staphylococcus aureus USA300 4 hour Log Control Agilent_252282510022_S01_GE2_107_Sep09_1_4 |
| GSM1904039 |
Staphylococcus aureus USA300 4 hour Log Control Agilent_252282510023_S01_GE2_107_Sep09_1_4 |
| GSM1904040 |
Staphylococcus aureus USA300 derived from Human Skin Pus Agilent_252282510024_S01_GE2_107_Sep09_1_3 |
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| Relations |
| BioProject |
PRJNA298051 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE73821_RAW.tar |
195.7 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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