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Status |
Public on Dec 28, 2015 |
Title |
Expression data for KDM1B knockdown in Glioma-Initiating Cells (GICs) |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Glioblastoma (GBM) is the most common and aggressive primary brain tumor in adults, with glioma initiating cells (GICs) implicated to be critical for tumor progression and resistance to therapy. KDM1B is involved in regulating GICs' responses to hypoxia, since over-expression of KDM1B delays the cell growth under hypoxia while knocking-down of KDM1B in GICs promotes their survival and tumorigenic abilities.
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Overall design |
We used RNA-Sequencing to detail the global change of gene expression in GICs with knockdown of KDM1B, and identified de-regulated genes and pathways downstream of KDM1B. CD133+ D456MG GICs were infected with non-targeting control and shRNA of KDM1B. Then RNA was extracted and gene expression was profiled by RNA-Seq.
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Contributor(s) |
Li H, Hu J, Wang X |
Citation(s) |
26766590 |
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Submission date |
Sep 29, 2015 |
Last update date |
May 15, 2019 |
Contact name |
Hai-Ri Li |
E-mail(s) |
hairili@ucsd.edu
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Phone |
858-534-4926
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Organization name |
University of California, San Diego
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Department |
Medicine
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Lab |
Dr. Francesca Telese
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Street address |
9500 Gilman Dr.
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City |
La Jolla |
State/province |
CA |
ZIP/Postal code |
92093 |
Country |
USA |
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Platforms (1) |
GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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Samples (6)
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Relations |
BioProject |
PRJNA297346 |
SRA |
SRP064317 |