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Series GSE73492 Query DataSets for GSE73492
Status Public on Nov 19, 2015
Title EWS and FUS Bind a Subset of Transcribed Genes Encoding Proteins Enriched in RNA Regulatory Functions
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Background FUS (TLS) and EWS (EWSR1) belong to the FET-protein family of RNA and DNA binding proteins. FUS and EWS are structurally and functionally related and participate in transcriptional regulation and RNA splicing. FUS and EWS are identified in translocation generated cancer fusion proteins and involved in the human neurological diseases amyotrophic lateral sclerosis and fronto-temporal lobar degeneration. Results To determine the gene regulatory functions of FUS and EWS at the level of chromatin, we have performed Chromatin Immunoprecipitation followed by Next Generation Sequencing (ChIP-Seq). Our results show that FUS and EWS bind to a subset of actively transcribed genes, that binding often is in the 3’ end of genes, and that binding overlaps with RNA polymerase II. Functional examinations of selected target genes verified that FUS and EWS can regulation expression. Gene Ontology (GO) analyses showed that FUS and EWS target genes preferentially encode proteins involved in regulatory processes at the RNA level. Conclusions The presented results yield new insights into gene interactions of EWS and FUS and have identified a set of FUS and EWS target genes involved in pathways at the RNA regulatory level with potential to mediate normal and disease-associated functions of the FUS and EWS proteins.
 
Overall design To identify the DNA binding profile of FUS, EWS and TAF15 by ChIPseq. Controls: use antibody against H3K9 and input
Web link http://www.biomedcentral.com/1471-2164/16/929/abstract
 
Contributor(s) Luo Y, Blechingberg J, Fernandes AM, Li S, Fryland T, Børglum AD, Bolund L, Nielsen AL
Citation(s) 26573619
Submission date Sep 28, 2015
Last update date May 15, 2019
Contact name Yonglun Luo
E-mail(s) yonglun.luo@hum-gen.au.dk
Phone 0045-89421666
Organization name Aarhus University
Street address Wilhelm Meyers Allé 4
City aarhus
ZIP/Postal code 8000
Country Denmark
 
Platforms (1)
GPL9052 Illumina Genome Analyzer (Homo sapiens)
Samples (5)
GSM1895985 FUS
GSM1895986 EWS
GSM1895987 TAF15
Relations
BioProject PRJNA297201
SRA SRP064249

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Supplementary file Size Download File type/resource
GSE73492_RAW.tar 1.6 Gb (http)(custom) TAR (of BED, TAR, XLS)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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