NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE73065 Query DataSets for GSE73065
Status Public on Sep 13, 2016
Title High-throughput single-base resolution mapping of 2’-O-Methylated residues
Organisms Homo sapiens; Mus musculus
Experiment type Other
Summary Functional characterization of the transcriptome requires tools for the systematic investigation of RNA post-transcriptional modifications. 2’-O-Methylation of the ribose moiety is one of the most abundant post-transcriptional modifications of the RNA. We describe here a high-throughput method that enables fast and accurate mapping at single-base resolution, and quantitation, of 2’-OMe modified residues. Our approach expands the actual repertoire of methods for transcriptome-wide mapping of RNA post-transcriptional modifications.
 
Overall design Mapping 2’-O-Methylated residues in Hela cells and E14 mouse embryonic stem cells.
 
Contributor(s) Incarnato D, Anselmi F, Morandi E, Neri F, Maldotti M, Rapelli S, Parlato C, Basile G, Oliviero S
Citation(s) 28180324
Submission date Sep 15, 2015
Last update date Mar 22, 2019
Contact name Danny Incarnato
E-mail danny.incarnato@hugef-torino.org
Organization name HuGeF
Department Epigenetics
Street address Via Nizza 52
City Torino
State/province Italy
ZIP/Postal code 10126
Country Italy
 
Platforms (3)
GPL15456 Illumina HiScanSQ (Homo sapiens)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (15)
GSM1881613 2OMe-seq 1mM dNTPs (HeLa #1, Method #1)
GSM1881614 2OMe-seq 0.004mM dNTPs (HeLa #1, Method #1)
GSM1881615 2OMe-seq 1mM dNTPs (HeLa, Method #2)
Relations
BioProject PRJNA295991
SRA SRP063871

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE73065_RAW.tar 570.0 Kb (http)(custom) TAR (of BEDGRAPH)
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap