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Series GSE72931 Query DataSets for GSE72931
Status Public on Oct 06, 2016
Title Bromodomain-containing Protein 4 (BRD4) is Required for the Maintenance of a Mammary Epithelial Phenotype [ChIP-Seq]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Bromodomain-containing protein 4 (BRD4) is an important epigenetic reader which promotes gene transcription to modulate cell-specific functions and is under intensive investigation for its potential as an anti-tumor therapeutic target. However, the role of BRD4 in non-transformed cells remains unclear. Here we demonstrate that BRD4 is required for the expression of epithelial-specific genes and suppression of stem cell-like properties by binding to the distal regions of epithelial-related genes. Moreover, BRD4 occupancy correlates with enhancer activity and enhancer RNA (eRNA) transcription of epithelial differentiation-specific genes. Interestingly, we show that BRD4 perturbation regulates the expression of Grainy Head-like transcription factor, GRHL3, whose depletion partially mimics BRD4 inhibition and blocks differentiated phenotype. By binding to the distal regions of GRHL3, BRD4 promotes RNA polymerase-II occupancy and thus affects eRNA transcription. Altogether, these findings provide evidence that BRD4 promotes a differentiated epithelial phenotype in non-transformed mammary cells at least in part through the activation of GRHL3 expression.
 
Overall design ChIP-sequencing of BRD4 and H3K27ac in MCF10A cells.
 
Contributor(s) Nagarajan S, Bedi U, Johnsen SA
Citation(s) 27980063
Submission date Sep 11, 2015
Last update date May 15, 2019
Contact name Steven A Johnsen
E-mail(s) sjohnsen@alumni.mayo.edu
Organization name University Medical Center Göttingen
Department Department of General, Visceral and Pediatric Surgery
Street address Robert-Koch-Straße 40
City Göttingen
State/province Niedersachsen
ZIP/Postal code 37075
Country Germany
 
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (5)
GSM1874947 BRD4 rep1
GSM1874948 BRD4 rep2
GSM1874949 BRD4 rep3
This SubSeries is part of SuperSeries:
GSE72933 Bromodomain-containing Protein 4 (BRD4) is Required for the Maintenance of a Mammary Epithelial Phenotype
Relations
BioProject PRJNA295370
SRA SRP063601

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE72931_BRD4.bigwig 760.5 Mb (ftp)(http) BIGWIG
GSE72931_H3K27ac.bigwig 678.3 Mb (ftp)(http) BIGWIG
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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