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| Status |
Public on May 01, 2017 |
| Title |
degradome sequence data |
| Organism |
Glycine max |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To identity the targets of miRNAs, we bundled 12 samples from different developing satages into four mixture samples. These samples were used to cosntruct degradome libraries and preform degradome sequencing on Illumina Hi-seq 2000 analyzer. More than 44.98 millions clean reads were obtained and 33.52 million reads were mapped to the soybean cDNA. The mapped reads were used to identity miRNA targets by CleaveLand4 pipeline.
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| |
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| Overall design |
4 degradome mixed samples, no replicates, but every degradome data consists of two parts data.
Please note that every degradome sample has two processed and two raw data files. To have enough data, additional sequencing was performed from each sample library. And each sample raw data was processed separately (tissue_name*degradome.txt) and also combined (all_degradome*.txt).
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| |
|
| Contributor(s) |
Tian Z |
| Citation(s) |
26714457 |
| |
| Submission date |
Sep 10, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Zhixi Tian |
| E-mail(s) |
zxtian@genetics.ac.cn
|
| Phone |
86-10-64803672
|
| Organization name |
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences
|
| Lab |
Zhixi Tian
|
| Street address |
No.1 Building, No.1 West Beichen Road,Chaoyang District
|
| City |
Beijing |
| ZIP/Postal code |
100101 |
| Country |
China |
| |
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| Platforms (1) |
| GPL15008 |
Illumina HiSeq 2000 (Glycine max) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA295299 |
| SRA |
SRP063566 |
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