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Series GSE72876 Query DataSets for GSE72876
Status Public on Dec 22, 2015
Title Expanding the CTD code: methylation of non-consensus Lysine residues marks early transcription in mammalian cells
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Dynamic post-translational modification of RNA polymerase II (RNAPII) coordinates the co-transcriptional recruitment of enzymatic complexes that regulate chromatin states and co-transcriptional processing of nascent RNA. Extensive phosphorylation of serine residues occurs at the structurally-disordered C-terminal domain (CTD) of the largest RNAPII subunit, which is composed of multiple heptapeptide repeats with consensus sequence Y1-S2-P3-T4-S5-P6-S7. Serine-5 and Serine-7 phosphorylation mark transcription initiation, whereas Serine-2 phosphorylation coincides with productive elongation. In vertebrates, the CTD has eight non-canonical substitutions of Serine-7 into Lysine-7, which can be acetylated (K7ac). Here, we describe for the first time mono- and di-methylation of CTD Lysine-7 residues (K7me1 and K7me2). K7me1 and K7me2 are observed during the earliest transcription stages and precede or accompany Serine-5 and Serine-7 phosphorylation.
Overall design Genome wide mapping of 2 novel RNAPII post-translational modifications (CTD-K7me1 and CTD-K7me2) in mouse ES cells.
Contributor(s) Dias JD, Rito T, Torlai Triglia E, Ferrai C, Kukalev A, Kimura H, Pombo A
Citation(s) 26687004
Submission date Sep 09, 2015
Last update date May 15, 2019
Contact name Elena Torlai Triglia
Organization name Max Delbrück Center for Molecular Medicine
Department BIMSB
Lab Ana Pombo Lab - Epigenetic Regulation and Genome Architecture
Street address Robert Rössle Str 10
City Berlin
State/province Deutschland
ZIP/Postal code 13125
Country Germany
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (2)
GSM1874007 CTD-K7me1 ChIP-seq
GSM1874008 CTD-K7me2 ChIP-seq
BioProject PRJNA295232
SRA SRP063538

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Supplementary file Size Download File type/resource
GSE72876_RAW.tar 560.1 Mb (http)(custom) TAR (of BW)
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Raw data are available in SRA
Processed data provided as supplementary file

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