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Series GSE72848 Query DataSets for GSE72848
Status Public on Sep 05, 2018
Title CARM1 Methylates MED12 to Regulate its RNA Binding Ability
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The coactivator-associated arginine methyltransferase (CARM1) functions as a regulator for transcription, splicing and chromatin regulation by methylating a diverse array of substrates. In this study, we used CARM1 substrate antibodies to perform immunoprecipitation coupled with mass spectrometry (IP-MS) in MEFs and identified Mediator Subunit 12 (MED12) as a novel substrate for CARM1. ChIP-seq analysis using CARM1, MED12 and H3R17me2a (represents ‘CARM1 activity’) antibodies revealed that MED12 targeted by CARM1 is recruited to the EREs (estrogen-responsive elements). Additionally, RT-PCR studies showed that the MED12R1899K mutation disrupting the methylation site reduced the expression of ER-target genes. Thus, MED12 methylation targets it to ER-specific enhancers and positively modulates transcription of Estrogen-driven genes.
Overall design ChIP-seq of CARM1, MED12 and H3R17me2a in MCF7 cells
Contributor(s) Cheng D, Lu Y, Vemulapalli V, Bedford M
Citation(s) 30456381
Submission date Sep 09, 2015
Last update date May 15, 2019
Contact name Yue Lu
Organization name MD Anderson Cancer Center
Department Epigenetics and Molecular Carcinogenesis
Street address 1808 Park Road 1C
City Smithville
State/province TX
ZIP/Postal code 78957
Country USA
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (4)
GSM1873366 CARM1
GSM1873367 MED12
GSM1873368 H3R17me2a
BioProject PRJNA295184
SRA SRP063514

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Supplementary file Size Download File type/resource
GSE72848_peaks.txt.gz 12.1 Kb (ftp)(http) TXT
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