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| Status |
Public on Oct 06, 2015 |
| Title |
Defining the consequences of genetic variation on a proteome-wide scale |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Genetic variation governs protein expression through both transcriptional and post-transcriptional processes. To investigate this relationship, we combined a multiplexed, mass spectrometry-based method for protein quantification with an emerging mouse model harboring extensive genetic variation from 8 founder strains. We collected genome-wide mRNA and protein profiling measurements to link genetic variation to protein expression differences in livers from 192 Diversity Outcross mice.
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| Overall design |
Illumina 100bp single-end liver RNA-seq from 192 male and female Diversity Outbred 26-week old mice raised on standard chow or high fat diet. Each sample was sequenced in 2x technical replicates across multiple flowcells. Samples were randomly assigned lanes and multiplexed at 12-24x.
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| Contributor(s) |
Munger SC, Chick JM, Simecek P, Huttlin EL, Choi K, Gatti DM, Raghupathy N, Svenson KL, Churchill GA, Gygi SP |
| Citation(s) |
27309819, 28592500 |
| |
| Submission date |
Sep 07, 2015 |
| Last update date |
Aug 28, 2019 |
| Contact name |
Steven C Munger |
| E-mail(s) |
steven.munger@jax.org
|
| Organization name |
The Jackson Laboratory
|
| Lab |
Steven Munger
|
| Street address |
600 Main Street
|
| City |
Bar Harbor |
| State/province |
ME |
| ZIP/Postal code |
04609 |
| Country |
USA |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (384)
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| Relations |
| BioProject |
PRJNA295057 |
| SRA |
SRP063455 |
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