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Series GSE72666 Query DataSets for GSE72666
Status Public on Mar 17, 2016
Title High-throughput sequence data of ChIP performed with anti-SMARCAD1 (ASNA) or anti-CBP actibodies.
Organism Drosophila melanogaster
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Histone acetylation and nucleosome remodeling play a pivotal role in transcriptional regulation. While histone acetylase and ATP-dependent chromatin remodeling activities have been well characterized, how the two activities are coordinated remains to be uncovered. We discovered ATP-dependent histone H2A acetylation activity in Drosophila nuclear extracts. This activity was column-purified and demonstrated to be composed of CBP and SMARCAD1, which belongs to the Etl1 subfamily of the Snf2 family of helicase-related proteins. SMARCAD1 enhanced acetylation of H2A K5 and K8 by CBP in nucleosomes in an ATP-dependent fashion. Expression array analysis of S2 cells having ectopically expressed SMARCAD1 revealed up-regulated genes. Using native genome templates of these up-regulated genes, we found that SMARCAD1 activates their transcription in vitro. Knockdown analysis of SMARCAD1 and CBP indicated overlapping gene control, and ChIP-seq analysis of these commonly controlled genes showed that CBP is recruited to the promoter prior to SMARCAD1. Moreover, Drosophila genetic experiments demonstrated interaction between SMARCAD1/Etl1 and CBP/nej during development. The interplay between the remodeling activity of SMARCAD1 and histone acetylation by CBP sheds light on chromatin and the genome-integrity network.
 
Overall design Examination of 2 different protein in 1 cell type.
 
Contributor(s) Ito T
Citation(s) 26888216
Submission date Sep 02, 2015
Last update date May 15, 2019
Contact name Masamichi Doiguchi
E-mail(s) doiguch@nagasaki-u.ac.jp
Organization name University of Nagasaki
Street address Sakamoto 1-12-4
City Nagasaki
ZIP/Postal code 852-8523
Country Japan
 
Platforms (1)
GPL16479 Illumina MiSeq (Drosophila melanogaster)
Samples (3)
GSM1867915 input DNA
GSM1867916 SMARCAD1 (ASNA) ChIP
GSM1867917 CBP ChIP
Relations
BioProject PRJNA294616
SRA SRP063273

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE72666_RAW.tar 760.0 Kb (http)(custom) TAR (of CSV, XLS)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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