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| Status |
Public on Mar 17, 2016 |
| Title |
High-throughput sequence data of ChIP performed with anti-SMARCAD1 (ASNA) or anti-CBP actibodies. |
| Organism |
Drosophila melanogaster |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Histone acetylation and nucleosome remodeling play a pivotal role in transcriptional regulation. While histone acetylase and ATP-dependent chromatin remodeling activities have been well characterized, how the two activities are coordinated remains to be uncovered. We discovered ATP-dependent histone H2A acetylation activity in Drosophila nuclear extracts. This activity was column-purified and demonstrated to be composed of CBP and SMARCAD1, which belongs to the Etl1 subfamily of the Snf2 family of helicase-related proteins. SMARCAD1 enhanced acetylation of H2A K5 and K8 by CBP in nucleosomes in an ATP-dependent fashion. Expression array analysis of S2 cells having ectopically expressed SMARCAD1 revealed up-regulated genes. Using native genome templates of these up-regulated genes, we found that SMARCAD1 activates their transcription in vitro. Knockdown analysis of SMARCAD1 and CBP indicated overlapping gene control, and ChIP-seq analysis of these commonly controlled genes showed that CBP is recruited to the promoter prior to SMARCAD1. Moreover, Drosophila genetic experiments demonstrated interaction between SMARCAD1/Etl1 and CBP/nej during development. The interplay between the remodeling activity of SMARCAD1 and histone acetylation by CBP sheds light on chromatin and the genome-integrity network.
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| Overall design |
Examination of 2 different protein in 1 cell type.
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| Contributor(s) |
Ito T |
| Citation(s) |
26888216 |
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| Submission date |
Sep 02, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Masamichi Doiguchi |
| E-mail(s) |
doiguch@nagasaki-u.ac.jp
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| Organization name |
University of Nagasaki
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| Street address |
Sakamoto 1-12-4
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| City |
Nagasaki |
| ZIP/Postal code |
852-8523 |
| Country |
Japan |
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| Platforms (1) |
| GPL16479 |
Illumina MiSeq (Drosophila melanogaster) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA294616 |
| SRA |
SRP063273 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE72666_RAW.tar |
760.0 Kb |
(http)(custom) |
TAR (of CSV, XLS) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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