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Series GSE72575 Query DataSets for GSE72575
Status Public on Nov 07, 2016
Title Expression data from glucosamine-treated human synovial MH7A cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Osteoarthritis (OA) is one of the major joint diseases. Glucosamine (GlcN) is widely used as a dietary supplement for OA. However, its precise mechanisms are not well understood. GlcN is utilized for the O-linked-N-acetylglucosamine (O-GlcNAc) modification of proteins, which participates in the regulation of cellular functions. In this study, we comprehensively analyzed the effect of GlcN and alloxan (O-GlcNAc transferase inhibitor, which prevented O-GlcNAc modification) on the gene expression using DNA microarray.
GlcN downregulated or upregulated genes. Furthermore, among the GlcN-downregulated or upregulated genes, the expression of 62.7% of the genes was restored by alloxan.. Thus, it is suggested that GlcN regulates the gene expression by an O-GlcNAc modification-dependent or -independent mechanism.
Overall design Human synovial MH7A cells in 6-well plates were incubated in the presence or absence of GlcN (5mM), and then stimulated with 15 pg/ml IL-1β for 13h. In some experiments, cells were preincubated with 5 mM alloxan, and then incubated with 2 mM alloxan and 5mM GlcN, followed by stimulation with IL-1β. We prepared 5 kinds of cell samples. 1; Non-treated cells. 2; IL-1β-stimulated cells. 3; GlcN-treated IL-1β-stimulated cells. 4; GlcN and alloxan-treated IL-1β-stimulated cells. 5; alloxan-treated IL-1β-stimulated cells. All expreriments were repeated three times.
Contributor(s) Someya A, Nagaoka I, Ikegami T, Sakamoto K
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Submission date Sep 01, 2015
Last update date Jul 26, 2018
Phone +81-3-5802-1033
Organization name Juntendo University
Department Department of Host Defense and Biochemical Research
Street address 2-1-1 Hongo
City Bunkyo-ku
State/province Tokyo
ZIP/Postal code 113-8421
Country Japan
Platforms (1)
GPL6244 [HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version]
Samples (15)
GSM1865425 MH7A-nontreated ex1
GSM1865426 MH7A IL-1β-stimulated ex1
GSM1865427 MH7A IL-1β-stimulated GlcN ex1
BioProject PRJNA294384

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Supplementary file Size Download File type/resource
GSE72575_RAW.tar 67.8 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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