|
| Status |
Public on Aug 29, 2016 |
| Title |
Follicular Helper T Cells Progressively Differentiate to Regulate the Germinal Center Response |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose: To compare the transcriptomes of IL-21-expressing, IL-21 and IL-4-expressing, and IL-4 expressing follicular helper T (Tfh) cells and Th2 cells in the spleen at 8 days following helminth infection Methods: Cell sorting of the populations was done for CD4+B220-CD44hiCXCR5hiPD-1hi cells of the various types, followed by mRNA purification.
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| |
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| Overall design |
CD4+Splenic T cell mRNA profiles 8 days post-infection of IL-21/IL-4 dual reporter mice with Nippostrongylus brasiliensis were generated by mRNA sequencing using Illumina HiSeq 2000.
|
| Web link |
http://dx.doi.org/10.1038/ni.3554
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| |
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| Contributor(s) |
Weinstein JS, Herman EI, Lainez B, Licona-Limón P, Esplugues E, Flavell R, Craft J |
| Citation(s) |
27573866 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| P30 AR053495 |
Rheumatic Diseases Research Core Centers: Administrative Core: Administrative Review: Core Center: Yale Rheumatic Disease Research Core Center: Yale Rheumatic Diseases Research Core Center |
YALE UNIVERSITY |
CRAFT |
| R37 AR040072 |
Immune Responses in Lupus |
YALE UNIVERSITY |
CRAFT |
| R21 AR063942 |
Manipulation of Follicular Helper T Cells in Immunity and Autoimmunity |
YALE UNIVERSITY |
CRAFT |
|
| Submission date |
Sep 01, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Edward Isaac Herman |
| E-mail(s) |
edward.herman@yale.edu
|
| Organization name |
Yale University
|
| Department |
School of Medicine
|
| Street address |
300 Cedar St, 541D TAC, PO Box 208031
|
| City |
New Haven |
| State/province |
Connecticut |
| ZIP/Postal code |
06520-8031 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
|
| Samples (8)
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| Relations |
| BioProject |
PRJNA294373 |
| SRA |
SRP063044 |
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