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Series GSE72337 Query DataSets for GSE72337
Status Public on Aug 09, 2016
Title Analysis of melanosomal miRNAs
Organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary miRNA microarray profiling was performed for MNT-1 cells, pre-mature and mature melanosomes extracted from MNT-1 cells. In order to reveal the miRNAs that are most abundant in mature melanosomes, the most highly expressed miRNAs from mature melanosomes were compared with expression levels in pre-mature melanosomes. The analysis revealed five miRNAs that were highly expressed in mature melanosomes and had been previously associated with melanoma.
 
Overall design Total RNA was extracted from MNT-1 cells (2 replicates), pre-mature and mature melanosomes. The melanosomes were either treated with RNase prior to RNA extraction (2 replicates each) or untreated (1 replicate each). Melanosomes were extracted from MNT-1 melanoma cells by sucrose-based density gradient ultracentrifugation. The treatment of melanosomes with RNase prior to RNA extraction was performed in order to remove any kind of RNA that may interact with or stick unspecifically to the outer side of the melanosome membrane. After 10 mins of RNAse treatment, RNase inhibitor was added. The samples of MNT-1 cells and untreated melanosomes were considered for the normalization, but not for further analyses.
Microarray Unit of the Core Facility Genomics and Proteomics DKFZ
 
Contributor(s) Sander L, Hoheisel JD, Dror S, Levy C
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Submission date Aug 24, 2015
Last update date Aug 09, 2016
Contact name Carmit Levy
E-mail(s) carmitlevy@post.tau.ac.il
Organization name Tel Aviv University
Department Human Genetics and Biochemistry
Lab Sackler School of Medicine
Street address Ramat Aviv
City Tel Aviv
ZIP/Postal code 69978
Country Israel
 
Platforms (1)
GPL19730 Agilent-046064 Unrestricted_Human_miRNA_V19.0_Microarray (Probe Name version)
Samples (8)
GSM1860444 MNT-1 cells_rep1
GSM1860445 MNT-1 cells_rep2
GSM1860446 Pre-mature melanosomes_untreated.rep1
This SubSeries is part of SuperSeries:
GSE72620 Melanoma miRNA trafficking triggers the tumor primary niche formation
Relations
BioProject PRJNA293786

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE72337_RAW.tar 26.9 Mb (http)(custom) TAR (of TXT)
Raw data provided as supplementary file
Processed data included within Sample table

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