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Series GSE72229 Query DataSets for GSE72229
Status Public on Aug 09, 2016
Title Expression profiling of fibroblasts treated with melanosomes
Organism Homo sapiens
Experiment type Expression profiling by array
Summary mRNA profiling was performed on primary fibroblasts treated with melanosomes and untreated fibroblasts as control. The aim of this study was to explore whether melanoma-derived mature melanosomes functionally affect primary fibroblasts. A functional gene set enrichment analysis based on the resulting mRNA profiles predicted that an uptake of mature melanosomes by fibroblasts causes increased cell proliferation and cell motility.
 
Overall design Expression profiling was performed for 3 replicates of primary human fibroblasts that were treated with mature melanosomes, 1 replicate of fibroblasts treated with early melanosomes and 3 replicates of untreated fibroblasts as controls. Melanosomes were extracted from the human melanoma cell line MNT-1. Fibroblasts were treated with melanosomes for 24h. Fold changes were calculed for fibroblasts treated with mature melanosomes vs. controls. The sample of fibroblasts treated with early melanosomes was considered for the normalization, but not for further analyses.
Microarray Unit of the Core Facility Genomics and Proteomics DKFZ
 
Contributor(s) Sander L, Hoheisel JD
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Submission date Aug 20, 2015
Last update date Aug 13, 2018
Contact name Carmit Levy
E-mail(s) carmitlevy@post.tau.ac.il
Organization name Tel Aviv University
Department Human Genetics and Biochemistry
Lab Sackler School of Medicine
Street address Ramat Aviv
City Tel Aviv
ZIP/Postal code 69978
Country Israel
 
Platforms (1)
GPL10558 Illumina HumanHT-12 V4.0 expression beadchip
Samples (7)
GSM1858138 Fibroblasts_untreated_rep1
GSM1858139 Fibroblasts_untreated_rep2
GSM1858140 Fibroblasts_untreated_rep3
This SubSeries is part of SuperSeries:
GSE72620 Melanoma miRNA trafficking triggers the tumor primary niche formation
Relations
BioProject PRJNA293440

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE72229_RAW.tar 37.4 Mb (http)(custom) TAR (of IDAT)
GSE72229_non-normalized_data.txt.gz 2.8 Mb (ftp)(http) TXT
Raw data provided as supplementary file
Processed data included within Sample table
Raw data are available on Series record

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