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Series GSE72172 Query DataSets for GSE72172
Status Public on Jan 09, 2016
Title Transcriptome analysis upon overexpression of SIN3 187HA in Drosophila cultured cells
Organism Drosophila melanogaster
Experiment type Expression profiling by high throughput sequencing
Summary SIN3 is a master transcriptional scaffold protein. SIN3 interacts with RPD3 and other accessory proteins to form a histone modifying complex. A single Sin3A gene encodes multiple isoforms of SIN3, of which SIN3 187 and SIN3 220 are the predominant isoforms. Previous studies demonstrated that SIN3 isoforms play non-redundant roles during fly development. In the current study, we sought to investigate the genes regulated by SIN3 187.
Overall design S2 cells and cells carrying a stable transgene of SIN3 187HA (SIN3 187HA cells) were treated with 0.07 ┬ÁM CuSO4. CuSO4 treatment led to ectopic expression of SIN3 187HA. S2 cells were used as a control. Following induction, total mRNA was extracted. mRNA profiling of these samples were performed by deep sequencing using Illumina Hiseq2500. Three biological replicates were performed.
Contributor(s) Saha N, Gajan A, Liu M, Pile LA
Citation(s) 26872827
Submission date Aug 18, 2015
Last update date May 15, 2019
Contact name Lori Ann Pile
Organization name Wayne State University
Department Biological Sciences
Street address 5047 Gullen Mall
City Detroit
State/province Michigan
ZIP/Postal code 48202
Country USA
Platforms (1)
GPL17275 Illumina HiSeq 2500 (Drosophila melanogaster)
Samples (6)
GSM1857008 S2 rep1
GSM1857009 S2 rep2
GSM1857010 S2 rep3
This SubSeries is part of SuperSeries:
GSE72173 Genome-wide studies reveal novel biological pathways regulated by SIN3 isoforms
SRA SRP062569
BioProject PRJNA294314

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Supplementary file Size Download File type/resource
GSE72172_S2_vs_SIN3_187.txt.gz 527.0 Kb (ftp)(http) TXT
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