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Series GSE71909 Query DataSets for GSE71909
Status Public on Jan 05, 2016
Title Bromodomain inhibition of the transcriptional coactivators CBP/EP300 as a therapeutic strategy to target the IRF4 network in multiple myeloma (ChIP-Seq)
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Pharmacological inhibition of chromatin co-regulatory factors represents a clinically validated strategy to modulate oncogenic signaling through selective attenuation of gene expression. Here, we demonstrate that CBP/EP300 bromodomain inhibition preferentially abrogates the viability of multiple myeloma cell lines. Phenotypic effects are preceded by the direct transcriptional suppression of the lymphocyte-specific transcription factor IRF4 and the subsequent down-regulation of the IRF4 transcriptional program. Ectopic expression of IRF4 antagonizes the phenotypic effects of CBP/EP300 bromodomain inhibition and prevents the suppression of the IRF4 target c-MYC. These findings suggest that CBP/EP300 bromodomain inhibition represents a viable therapeutic strategy for targeting multiple myeloma and other lymphoid malignancies dependent on the IRF4 network.
 
Overall design A total of 13 ChIP-seq samples were sequenced. Samples were treated with control (DMSO) or test compound (2.5 uM SGC-CBP30 or 0.25uM CPI267203) for 6 hours. Signal from input samples was included to subtract background signal from each ChIP-seq sample. Antibodies used were against p300, H3K18ac, H3K27ac, or BRD4.
 
Contributor(s) Conery AR, Centore RC, Hatton C, Bryant B, Sims III RJ
Citation(s) 26731516
Submission date Aug 10, 2015
Last update date May 15, 2019
Contact name Barbara M Bryant
E-mail(s) barbara.bryant@constellationpharma.com
Organization name Constellation Pharmaceuticals
Street address 215 First Street
City Cambridge
State/province MA
ZIP/Postal code 02142
Country USA
 
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (13)
GSM1847311 LP1_DMSO_P300
GSM1847312 LP1_SGC-CBP30_P300
GSM1847313 LP1_DMSO_H3K18ac
This SubSeries is part of SuperSeries:
GSE71911 Bromodomain inhibition of the transcriptional coactivators CBP/EP300 as a therapeutic strategy to target the IRF4 network in multiple myeloma
Relations
BioProject PRJNA292437
SRA SRP062237

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE71909_RAW.tar 9.7 Gb (http)(custom) TAR (of TDF, WIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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