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Series GSE71845 Query DataSets for GSE71845
Status Public on Jan 15, 2016
Title Oyster reproduction is affected by exposure to polystyrene microplastics
Organism Crassostrea gigas
Experiment type Expression profiling by array
Summary Plastics are persistent synthetic polymers that accumulate in the marine environment as waste. Microplastic (MP) particles are derived from the breakdown of larger debris or can enter the environment as microscopic fragments. Filter-feeder organisms ingest MP while feeding and are likely to be impacted by MP pollution. To assess the impact of polystyrene microspheres (PS) on the physiology of the Pacific oyster, adult oysters were experimentally exposed to virgin micro-PS (2 and 6 µm in diameter; 32 µg L-1) for two months during a reproductive cycle. Effects were investigated on transcriptomic responses, in digestive gland gonads and oocytes of exposed oysters.
Transcriptomic profiles in the tissues of the exposed oyster showed endocrine disrupting signals, notably highlighting alteration in glucocorticoid response, insulin pathway and fatty-acid metabolism in response to micro-PS exposition. In oocytes from exposed females, several transcripts coding for proteins involved in Ca2+ binding were differentially expressed suggesting a disruption of the Ca2+ signaling pathway with crucial consequences on oocyte maturation.
 
Overall design To assess the impact of polystyrene microspheres (PS) on the physiology of the Pacific oyster, adult oysters were experimentally exposed to virgin micro-PS (2 and 6 µm in diameter; 32 µg L-1) for two months during a reproductive cycle and compared to control oysters.
Adults were sampled 2 and 8 weeks after the beginning of exposure (corresponding to T1 and T3, respectively, 8-9 replicates per time of sampling and condition for a total of 56). Tissues were immediately dissected from each oyster, frozen in liquid nitrogen, then crushed to a fine powder at -196°C with an oscillating mill mixer and stored in liquid nitrogen until RNA extraction.
Oocytes were collected from 5 females per condition, filtered in a 40 µm sieve, counted and transferred into 1.5 mL of Extract-all reagent (Eurobio, Courtaboeuf, France) (20,000 oocytes).
Total RNA was isolated using 1.5 mL of Extract-all Reagent per 50 mg of gonad powder. For microarray hybridizations, 200 ng of total RNA were indirectly labeled with Cy3 using the Low Input Quick Amp Labeling kit One-Color. Hybridization was performed using the Agilent Gene expression hybridization kit (5188-5242), with 1.65 μg of labeled RNA, for 16 h at 65 °C. The employed arrays were Agilent 60-mer 4x44K custom microarrays, containing 31,918 C. gigas ESTs, designed by Dheilly et al. (2011). Slides were scanned on an Agilent Technologies G2565AA Microarray Scanner system at 5 μm resolution, using default parameters. Features were extracted using the Agilent Feature Extraction software 6.1.
 
Contributor(s) Sussarellu R, Suquet M, Yoann T, Lambert C, Fabioux C, Arsenault-Pernet ME, Le Goic N, Quillien V, Mingant C, Paul-Pont I, Epelboin Y, Corporeau C, Guyomarch J, Robbens J, Soudant P, Huvet A
Citation(s) 26831072
Submission date Aug 07, 2015
Last update date Apr 01, 2016
Contact name Rossana Sussarellu
E-mail rossana.sussarellu@ifremer.fr
Organization name Ifremer
Department Ressources Biologiques et Environnement
Lab Laboratoire d'Ecotoxicologie
Street address Rue de l'Ile d'Yeu
City Nantes
ZIP/Postal code 44300
Country France
 
Platforms (1)
GPL11353 Oyster Crassostrea gigas microarray v1
Samples (59)
GSM1846684 D_T1M25
GSM1846685 D_T1M26
GSM1846686 D_T1M33
Relations
BioProject PRJNA292304

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Supplementary file Size Download File type/resource
GSE71845_RAW.tar 276.5 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data provided as supplementary file
Processed data included within Sample table

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