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Series GSE71751 Query DataSets for GSE71751
Status Public on Apr 06, 2016
Title GATA3 restrains Notch signaling activity and represses NK cell development to induce human T cell commitment following Notch-induced T-lineage specification (ChIP-Seq)
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The stepwise conversion of multipotent precursors into committed T-cell progenitors depends on several transcriptional regulators, but the interplay between these factors is still obscure. This is particularly true in human since the core early Notch signalling pathway also supports NK cell development and requires tight regulation for efficient T-lineage commitment and differentiation. Here, we show that GATA3, in contrast to TCF1, induces T-lineage commitment following NOTCH1-induced T-lineage specification through direct regulation of at least 3 distinct processes: repression of NK-cell fate, activation of T-lineage genes to promote further differentiation, and downmodulation of Notch signalling activity. GATA3-mediated repression of the NOTCH1 target gene DTX1 hereby is essential to induce T-lineage commitment at the expense of NK cell differentiation. Thus, human T-lineage commitment is dependent on the precise collaboration of several transcriptional regulators that integrate through both positive and negative regulatory loops.
 
Overall design ChIP-sequencing data was generated for GATA3 in human thymocytes
 
Contributor(s) Van de Walle I, Durinck K, Van Loocke W, Damle S, Van Vlierberghe P, Speleman F, Rothenberg E, Taghon T
Citation(s) 27048872
Submission date Aug 05, 2015
Last update date May 15, 2019
Contact name Inge Van de Walle
E-mail(s) i.vandewalle@ugent.be
Organization name UGent
Department GE06
Lab Experimental Immunology
Street address De Pintelaan 185, blokA 4th floor
City Ghent
ZIP/Postal code 9000
Country Belgium
 
Platforms (1)
GPL9115 Illumina Genome Analyzer II (Homo sapiens)
Samples (3)
GSM1844609 input sample total thymocytes
GSM1844610 GATA3 ChIP total thymocytes replicate 1
GSM1844611 GATA3 ChIP total thymocytes replicate 2
This SubSeries is part of SuperSeries:
GSE71753 GATA3 restrains Notch signaling activity and represses NK cell development to induce human T cell commitment following Notch-induced T-lineage specification
Relations
BioProject PRJNA292009
SRA SRP062065

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Supplementary file Size Download File type/resource
GSE71751_RAW.tar 980.0 Kb (http)(custom) TAR (of BED, TXT, XLS)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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