|
| Status |
Public on Aug 06, 2015 |
| Title |
Molecular hydrogen stimulates the gene expression of a translational coactivator PGC-1alpha to enhance fatty acid metabolism |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
We previously reported that molecular hydrogen (H2) acts as a novel antioxidant to exhibits multiple-functions. Moreover, long-term drinking of H2-water (water infused with H2) enhanced energy expenditure to improve obesity and diabetes in db/db mice, accompanied with the increased gene expression of an energy metabolism stimulatory hepatic hormone, fibroblast growth factor 21 (FGF21); however, the molecular mechanism is unknown particularly on whether this gene regulation is a primary cause or the secondary consequence. Pathway analyses based on comprehensive gene expression revealed the increased expression in various genes involved in fatty acid and steroid metabolisms. As a transcription pathway, the PPARa signaling pathway was identified to up-regulate the genes by 14 day-ingestion of H2. As an early event, the gene expression of PGC-1a was transiently increased at day 3, followed by increased expression of FGF21. In wild-type mice that fed with fatty-diet, H2-water improved the level of plasma triglyceride and extended their life span.
|
| |
|
| Overall design |
H2 was ingested by ad libitum drinking H2-water for 14 days or by oral administration of an H2-producing material MgH2 for 1, 3 or 7 days. Comprehensive gene expression profile in liver of db/db mice was analyzed by DNA microarray.
|
| |
|
| Contributor(s) |
Kamimura N, Ichimiya H, Ohta S |
| Citation(s) |
28721265 |
| |
| Submission date |
Aug 05, 2015 |
| Last update date |
Jul 11, 2019 |
| Contact name |
Shigeo Ohta |
| E-mail(s) |
ohta@nms.ac.jp
|
| Phone |
+81-44-733-9267
|
| Organization name |
Institute of Development and Aging Sciences, Graduate School of Medicine, Nippon Medical School
|
| Department |
Department of Biochemistry and Cell Biology
|
| Street address |
1-394, Kosugi-machi, Nakahara-ku
|
| City |
Kawasaki-city |
| State/province |
Kanagawa |
| ZIP/Postal code |
211-8533 |
| Country |
Japan |
| |
|
| Platforms (1) |
| GPL10333 |
Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Feature Number version) |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA291974 |
Less...
More...