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Series GSE71733 Query DataSets for GSE71733
Status Public on Aug 06, 2015
Title Immortalized human hepatocyte (IHH) microRNA response to interferon alpha treatment
Organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary The purpose of this experiment was to obtain samples for microRNA analysis in IHH cells infected with human interferon alpha.
Overall design Immortalized human hepatocyte (IHH) cells were seeded at 1 x 10^6 cells per well on 6 well plates 3 days before infection. Treatments were initiated by replacing culture medium (containing 10% FBS) with fresh medium containing 500 U/ml of human interferon α (PBL Biosciences). Mock-treated cells were given normal culture medium only. Mock and treated cells were incubated at 37°C until sample collection time points. Infected samples were collected in triplet; time-matched mocks were collected in triplet in parallel with infected samples. Time points: 6, 12, and 18 h post-infection
Contributor(s) Kawaoka Y, Waters KM, Halfmann P, Eisfeld AJ
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Submission date Aug 04, 2015
Last update date Aug 31, 2016
Contact name Natalie Heller
Organization name PNNL
Street address 902 Battelle Blvd.
City Richland
ZIP/Postal code 99354
Country USA
Platforms (1)
GPL19730 Agilent-046064 Unrestricted_Human_miRNA_V19.0_Microarray (Probe Name version)
Samples (16)
GSM1844324 microRNA_IFNaIHH001_Mock_6h_1
GSM1844325 microRNA_IFNaIHH001_Mock_6h_2
GSM1844326 microRNA_IFNaIHH001_Mock_6h_3
This SubSeries is part of SuperSeries:
GSE65575 Modeling Host Responses to Understand Severe Human Virus Infections
BioProject PRJNA292000

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE71733_RAW.tar 52.0 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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