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Series GSE71732 Query DataSets for GSE71732
Status Public on Aug 06, 2015
Title Immortalized human hepatocyte (IHH) mRNA response to interferon alpha treatment
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The purpose of this experiment was to obtain samples for mRNA analysis in IHH cells infected with human interferon alpha.
 
Overall design Immortalized human hepatocyte (IHH) cells were seeded at 1 x 10^6 cells per well on 6 well plates 3 days before infection. Treatments were initiated by replacing culture medium (containing 10% FBS) with fresh medium containing 500 U/ml of human interferon α (PBL Biosciences). Mock-treated cells were given normal culture medium only. Mock and treated cells were incubated at 37°C until sample collection time points. Infected samples were collected in triplet; time-matched mocks were collected in triplet in parallel with infected samples. Time points: 6, 12, and 18 h post-infection
 
Contributor(s) Kawaoka Y, Waters KM, Halfmann P, Eisfeld AJ
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Submission date Aug 04, 2015
Last update date Jan 09, 2018
Contact name Natalie Heller
E-mail natalie.heller@pnnl.gov
Organization name PNNL
Street address 902 Battelle Blvd.
City Richland
ZIP/Postal code 99354
Country USA
 
Platforms (1)
GPL13497 Agilent-026652 Whole Human Genome Microarray 4x44K v2 (Probe Name version)
Samples (17)
GSM1844307 IFNaIHH001_Mock_6h_1
GSM1844308 IFNaIHH001_Mock_6h_2
GSM1844309 IFNaIHH001_Mock_6h_3
This SubSeries is part of SuperSeries:
GSE65575 Modeling Host Responses to Understand Severe Human Virus Infections
Relations
BioProject PRJNA291999

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE71732_RAW.tar 36.8 Mb (http)(custom) TAR (of TXT)
Raw data provided as supplementary file
Processed data included within Sample table

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