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Series GSE71726 Query DataSets for GSE71726
Status Public on Aug 06, 2015
Title Whole rye consumption improves blood and liver n-3 fatty acid profile and gut microbiota composition in rats
Platform organism human gut metagenome
Sample organism uncultured bacterium
Experiment type Expression profiling by array
Summary This study in rats was designed to investigate whether whole rhye (WR) can influence the metabolism of n-3 and n-6 long-chain fatty acids (LCFA) and gut microbiota composition. For 12 weeks, rats were fed a diet containing either 50% WR or 50% refined rye (RR). Total bacterial DNA was extracted from fecal and cecal samples (n=5 per group). 16S PCR amplification was performed to assess the microbial diversity at the family level using the HuGChip. Amplified DNA was purified and labelled with either Cy3 or Cy5 dye and hybridized on the microarray.
 
Overall design A 15 chip study was realized, each corresponding to hybridization with 250ng of labelled 16S rRNA gene amplicons from either mice fecal and cecal samples. Each probe (4441) was synthetized in three replicates.
 
Contributor(s) Ounnas F, Privé F, Salen P, Gaci N, Tottey W, Calani L, Bresciani L, López-Gutiérrez N, Hazane-Puch F, Laporte F, Melegari C, Brugère J, Del Rio D, Demeilliers C, de Lorgeril M
Citation(s) 26862900
Submission date Aug 04, 2015
Last update date Oct 07, 2019
Contact name william tottey
E-mail(s) williamtottey@gmail.com
Organization name EA-CIDAM 4678
Street address 28 place Henry Dunant
City Clermont-Ferrand
ZIP/Postal code 63000
Country France
 
Platforms (1)
GPL20773 Agilent-040558
Samples (30)
GSM1843854 250ng 16S rRNA gene amplicon of mouse faecal sample t0_SC5
GSM1843855 250ng 16S rRNA gene amplicon of mouse faecal sample t12_SR1
GSM1843856 250ng 16S rRNA gene amplicon of mouse faecal sample t0_SC7
Relations
BioProject PRJNA291983

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE71726_RAW.tar 38.3 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table
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