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Series GSE7164 Query DataSets for GSE7164
Status Public on Jul 31, 2007
Title Identification of novel genes expressed during mouse molar tooth development by microarray gene expression analysis
Organism Mus musculus
Experiment type Expression profiling by array
Summary To identify genes heretofore undiscovered as critical players in the biogenesis of teeth, we have used microarray gene expression analysis of the developing mouse molar tooth (DMT) between 1 and 10 days postnatal to identify genes differentially expressed when compared to 16 control tissues (GEO accession # GSE1986). Of the top 100 genes exhibiting increased expression in the DMT, 29 were found to have been previously associated with tooth development. Differential expression of the remaining 71 genes not previously associated with tooth development was confirmed by qRT-PCR analysis. Further analysis of seven of the latter genes by mRNA in situ hybridization found that five were specific to the developing tooth in the craniofacial region (Rspo4, Papln, Amtn, Gja1, Maf). Of the remaining two, one was found to be more widely expressed (Sp7) and the other was found to be specific to the nasal serous gland, which is close to, but distinct from, the developing tooth (Vrm).
Keywords: tooth development
 
Overall design mRNA from molar teeth extracted from Swiss Webster mouse pups between 1 and 10 days post-natal was pooled, labeled, and hybridized in quadruplicate to Affymetrix Mouse Genome Expression 430 2.0 microarrays. This data was compared to that of 16 control tissues (GEO accession # GSE1986) to identify genes differentially expressed in the DMT mRNA.
 
Contributor(s) Pemberton TJ, Mendoza-Fandino GA, Hsu Y, Snead ML, Mehrian-Shai R, Patel PI
Citation(s) 17626284
Submission date Feb 28, 2007
Last update date Feb 11, 2019
Contact name Trevor J Pemberton
E-mail(s) trevorjp@umich.edu
Phone (734)615-9551
Fax (734)615-6553
Organization name University of Michigan
Department Human Genetics
Lab Center for Computational Medicine and Biology
Street address 100 Washtenaw Ave
City Ann Arbor
State/province MI
ZIP/Postal code 48109
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (4)
GSM172512 Dental Molar Tooth (DMT) replicate 1
GSM172513 Dental Molar Tooth (DMT) replicate 2
GSM172514 Dental Molar Tooth (DMT) replicate 3
Relations
BioProject PRJNA98171

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE7164_RAW.tar 16.6 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data provided as supplementary file

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