NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE71621 Query DataSets for GSE71621
Status Public on Oct 01, 2015
Title Cell type-specific chromatin states differentially prime squamous cell carcinoma tumor-initiating cells for epithelial to mesenchymal transition
Organism Mus musculus
Experiment type Expression profiling by array
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary Epithelial to mesenchymal transition (EMT) in cancer cells has been associated with metastasis, stemness and resistance to therapy. The reason why some tumors undergo EMT and other not might reflect intrinsic properties of their cell of origin, although this possibility is largely unexplored. By targeting the same oncogenic mutations to discrete skin compartments, we show cell type-specific chromatin and transcriptional states differentially prime tumors to EMT. Squamous cell carcinomas (SCCs) derived from intrafollicular epidermis (IFE) are generally well-differentiated, while hair follicle (HF) stem cell-derived SCCs frequently exhibit EMT, efficiently form secondary tumors, and possess increased metastatic potential. Transcriptional and epigenomic profiling revealed IFE and HF tumor-initiating cells possess distinct chromatin landscapes and gene regulatory networks associated with tumorigenesis and EMT that correlate with accessibility of key epithelial and EMT transcription factor binding sites. These findings highlight the importance of chromatin states and transcriptional priming in dictating tumor phenotypes and EMT.
 
Overall design Refer to individual Series
 
Contributor(s) Latil ML, Nassar D, Beck B, Boumhadi S, Wang L, Brisebarre A, Yi R, Blanpain C
Citation(s) 27889319
Submission date Jul 31, 2015
Last update date Mar 19, 2019
Contact name C├ędric Blanpain
E-mail cedric.blanpain@ulb.ac.be
Organization name ULB
Street address Route de Lennik 808
City Brussels
ZIP/Postal code 1070
Country Belgium
 
Platforms (4)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
GPL11180 [HT_MG-430_PM] Affymetrix HT MG-430 PM Array Plate
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (40)
GSM1782563 HF_normal_single-end
GSM1782564 HF_normal_paired-end
GSM1782565 IFE_normal_single-end
This SuperSeries is composed of the following SubSeries:
GSE70474 Cell type-specific chromatin states differentially prime squamous cell carcinoma tumor-initiating cells for epithelial to mesenchymal transition [ATAC-seq]
GSE87877 Cell type-specific chromatin states differentially prime squamous cell carcinoma tumor-initiating cells for epithelial to mesenchymal transition [expression 1]
GSE88762 Cell type-specific chromatin states differentially prime squamous cell carcinoma tumor-initiating cells for epithelial to mesenchymal transition [expression 2]
Relations
BioProject PRJNA291632

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE71621_RAW.tar 68.8 Mb (http)(custom) TAR (of CEL, TXT)

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap