|
| Status |
Public on May 22, 2016 |
| Title |
Effects of biobanking on chromatin accessibility |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
Other
|
| Summary |
The goal of this study was to test the effects of biobanking on chromatin assessibility, as defined by ATAC-seq. Human naïve B cells were FACS sorted from PBMCs that were either frozen or processed fresh. A range of cell numbers were used to identify technical limitations and reproducibility of the ATAC-seq assay on biobanked PBMCs comapred to freshly sorted material. To provide insight into a disease process, naive B cells were isolated from healthy donors or a biorepository of Systemic Lupus Erythematosus undergoing disease flares. These data identify a unique signature of accessible chromatin in SLE B cells.
|
| |
|
| Overall design |
ATAC-Seq on fresh and frozen B cells
|
| |
|
| Contributor(s) |
Scharer CD, Boss J |
| Citation(s) |
27249108 |
| |
| Submission date |
Jul 24, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Chris Scharer |
| E-mail(s) |
cdschar@emory.edu
|
| Organization name |
Emory University
|
| Department |
Microbiology and Immunology
|
| Lab |
Chris Scharer
|
| Street address |
1510 Clifton Rd, Suite 3086A
|
| City |
Atlanta |
| State/province |
GA |
| ZIP/Postal code |
30322 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (16)
|
|
| Relations |
| BioProject |
PRJNA290920 |
| SRA |
SRP061568 |
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