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| Status |
Public on Mar 16, 2016 |
| Title |
Transcriptome assay of materal mRNA degradation in Btg4-deleted oocytes |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We analyzed the functions of BTG family proteins in maternal mRNA degradation in mouse oocytes. By comparing the degradation of transcripts in WT oocytes and KO oocytes, we are able to know the defects in maternal mRNA clearance in BTG4-deleted oocytes, and identified the BTG4 target genes in oocyte cyplasmic maturation.
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| Overall design |
2 WT oocyte samples at GV stage, 2 WT oocyte samples at MII stage, 2 Btg4-/- oocyte samples at GV stage and 2 Btg4-/- oocyte samples at MII stage?2 WT embryo samples at zygote stage, 2 WT embryo samples at 2-cell stage, 2 Btg4-/- embryo samples at zygote stage and 2 Btg4-/- embryo samples at 2-cell stage , and a WT GV oocyte, a WT MII oocyte, a Erk-/- GV oocyte and a Erk-/- MII oocyte are performed RNA sequencing.
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| Contributor(s) |
Yu C, Ji S, Dang Y |
| Citation(s) |
27065194 |
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| Submission date |
Jul 23, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
yujiao dang |
| E-mail(s) |
1101110321@pku.edu.cn
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| Organization name |
BIOPIC
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| Street address |
Yihe road
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| City |
beijing |
| ZIP/Postal code |
100871 |
| Country |
China |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (20)
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| Relations |
| BioProject |
PRJNA290718 |
| SRA |
SRP061454 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE71257_RAW.tar |
14.2 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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