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Series GSE71257 Query DataSets for GSE71257
Status Public on Mar 16, 2016
Title Transcriptome assay of materal mRNA degradation in Btg4-deleted oocytes
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We analyzed the functions of BTG family proteins in maternal mRNA degradation in mouse oocytes. By comparing the degradation of transcripts in WT oocytes and KO oocytes, we are able to know the defects in maternal mRNA clearance in BTG4-deleted oocytes, and identified the BTG4 target genes in oocyte cyplasmic maturation.
 
Overall design 2 WT oocyte samples at GV stage, 2 WT oocyte samples at MII stage, 2 Btg4-/- oocyte samples at GV stage and 2 Btg4-/- oocyte samples at MII stage?2 WT embryo samples at zygote stage, 2 WT embryo samples at 2-cell stage, 2 Btg4-/- embryo samples at zygote stage and 2 Btg4-/- embryo samples at 2-cell stage , and a WT GV oocyte, a WT MII oocyte, a Erk-/- GV oocyte and a Erk-/- MII oocyte are performed RNA sequencing.
 
Contributor(s) Yu C, Ji S, Dang Y
Citation(s) 27065194
Submission date Jul 23, 2015
Last update date May 15, 2019
Contact name yujiao dang
E-mail 1101110321@pku.edu.cn
Organization name BIOPIC
Street address Yihe road
City beijing
ZIP/Postal code 100871
Country China
 
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (20)
GSM1831454 BTG4ko_GV1
GSM1831455 BTG4ko_GV2
GSM1831456 WT_GV1
Relations
BioProject PRJNA290718
SRA SRP061454

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE71257_RAW.tar 14.2 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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