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Status |
Public on Jul 17, 2016 |
Title |
Global gene expression analysis between cetuximab-sensitive and cetuximab-resistant colorectal cancer cells |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
Cetuximab is an epidermal growth factor receptor (EGFR)-blocking antibody approved for treatment of metastatic colorectal cancer. We examined differences in global gene expression between the syngeneic DiFi colorectal cancer cells, and a subline of DiFi cells with acquired resistance to cetuximab (DiFi5). We used Affymetrix HG-U133A array to compare the expression pattern of genes that are up-regulated or down-regulated between the parental DiFi colorectal cancer cells and the cetuximab-resistant DiFi5 cells.
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Overall design |
We generated a cetuximab-resistant DiFi subline, termed DiFi5, by chronic exposure of parental DiFi cells to serially increased doses of cetuximab (from 0.5 nM to 5 nM) for over 1 year. The resulting DiFi5 subline exhibits significant resistance to cetuximab-induced apoptosis. After we confirmed that the phenotype was stable over a period of time of more than 6 months, the RNA from DiFi and DiFi5 cells were extracted and hybridized to an Affymetrix HG-U133A array according to the manufacturer’s instructions. Following the hybridization, the array was scanned using a laser confocal scanner, and microarray image data were analyzed using DNA-Chip Analyzer (dChip), version 1.3, by the Sequencing and Microarray Facility at MD Anderson Cancer Center.
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Contributor(s) |
Lu Y, Fan Z |
Citation missing |
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Submission date |
Jul 22, 2015 |
Last update date |
Aug 10, 2018 |
Contact name |
Zhen Fan |
E-mail(s) |
fanzhen8@gmail.com
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Phone |
7137453560
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Organization name |
UT MD Anderson Cancer Center
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Department |
Experimental Therapeutics
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Lab |
Fan
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Street address |
1515 Holcombe Blvd.
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City |
Houston |
State/province |
TX |
ZIP/Postal code |
77030 |
Country |
USA |
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Platforms (1) |
GPL96 |
[HG-U133A] Affymetrix Human Genome U133A Array |
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Samples (2) |
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Relations |
BioProject |
PRJNA290584 |