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Series GSE71018 Query DataSets for GSE71018
Status Public on Feb 25, 2016
Title Grainyhead-like 2 (GRHL2) maintains the epithelial status of ovarian cancer through miR-200-ZEB1 regulatory networks (ChIP-Seq)
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Epithelial ovarian cancer (EOC) is clinically heterogeneous, comprising different histological and biological subtypes. Multiple studies have implicated epithelial-mesenchymal transition (EMT), a biological process by which polarized epithelial cells convert into a mesenchymal phenotype, to contribute significantly to this molecular heterogeneity of EOC. From gene expression analyses of a collection of EMT-characterized EOC cell lines, we found that the expression of the transcription factor Grainyhead-like 2 (GRHL2) correlates with E-cadherin expression and the epithelial phenotype. EOC tumors with lower levels of GRHL2 are associated with the Mes (mesenchymal) molecular subtype and show poorer overall survival in patients. Here, we demonstrate that shRNA-mediated knockdown of GRHL2 in EOC cells with an epithelial phenotype resulted in EMT changes, with increased cell migration, invasion and motility. By ChIP-sequencing and gene expression microarray, we identified a variety of target genes regulated by GRHL2, including protein-coding and non-coding genes. Our data suggest that GRHL2 maintains the epithelial phenotype of EOC cells through the regulatory networks of miR-200b/a, ZEB1 and E-cadherin. These findings support GRHL2 as a crucial player in the molecular heterogeneity of EOC.
Overall design 3 samples were analyzed (GRHL2 ChIP sample for 3 cell lines)
Contributor(s) Chung V, Tan T, Tan M, Wong M, Kuay K, Yang Z, Ye J, Muller J, Koh C, Guccione E, Thiery J, Huang R
Citation(s) 26887977
Submission date Jul 16, 2015
Last update date May 15, 2019
Contact name Tuan Zea Tan
Organization name National University of Singapore
Department Cancer Science Institute Singapore
Street address 14 Medical Drive, #11-01, Singapore
City Singapore
ZIP/Postal code 117599
Country Singapore
Platforms (1)
GPL9052 Illumina Genome Analyzer (Homo sapiens)
Samples (6)
GSM1825673 OVCAR3-inputDNA
GSM1825674 OVCA429-inputDNA
GSM1825675 PEO1-inputDNA
This SubSeries is part of SuperSeries:
GSE71019 Grainyhead-like 2 (GRHL2) maintains the epithelial status of ovarian cancer through miR-200-ZEB1 regulatory networks
BioProject PRJNA290109
SRA SRP061246

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE71018_ovcar3_grhl2_macs2_s25_e5_pw200_latest_ucsc-refGene_peaklist-1.xlsx 257.4 Kb (ftp)(http) XLSX
GSE71018_ovcar429_grhl2_macs2_s25_e5_pw200_latest_ucsc-refGene_peaklist.xlsx 1.4 Mb (ftp)(http) XLSX
GSE71018_peo1_grhl2_macs2_s25_e5_pw200_latest_ucsc-refGene_peaklist.xlsx 1007.4 Kb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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